Appalachian State University, Human Performance Lab, North Carolina Research Campus, Kannapolis, North Carolina;
Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China; and.
Am J Physiol Regul Integr Comp Physiol. 2014 Jul 1;307(1):R68-74. doi: 10.1152/ajpregu.00092.2014. Epub 2014 Apr 23.
Bioactive oxidized linoleic acid metabolites (OXLAMs) include 13- and 9-hydroxy-octadecadienoic acid (13-HODE + 9-HODE) and have been linked to oxidative stress, inflammation, and numerous pathological and physiological states. The purpose of this study was to measure changes in plasma 13-HODE + 9-HODE following a 75-km cycling bout and identify potential linkages to linoleate metabolism and established biomarkers of oxidative stress (F2-isoprostanes) and inflammation (cytokines) using a metabolomics approach. Trained male cyclists (N = 19, age 38.0 ± 1.6 yr, wattsmax 304 ± 10.5) engaged in a 75-km cycling time trial on their own bicycles using electromagnetically braked cycling ergometers (2.71 ± 0.07 h). Blood samples were collected preexercise, immediately post-, 1.5 h post-, and 21 h postexercise, and analyzed for plasma cytokines (IL-6, IL-8, IL-10, tumor necrosis factor-α, monocyte chemoattractant protein-1, granulocyte colony-stimulating factor), F2-isoprostanes, and shifts in metabolites using global metabolomics procedures with gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS). 13-HODE + 9-HODE increased 3.1-fold and 1.7-fold immediately post- and 1.5 h postexercise (both P < 0.001) and returned to preexercise levels by 21-h postexercise. Post-75-km cycling plasma levels of 13-HODE + 9-HODE were not significantly correlated with increases in plasma cytokines but were positively correlated with postexercise F2-isoprostanes (r = 0.75, P < 0.001), linoleate (r = 0.54, P = 0.016), arachidate (r = 0.77, P < 0.001), 12,13-dihydroxy-9Z-octadecenoate (12,13-DiHOME) (r = 0.60, P = 0.006), dihomo-linolenate (r = 0.57, P = 0.011), and adrenate (r = 0.56, P = 0.013). These findings indicate that prolonged and intensive exercise caused a transient, 3.1-fold increase in the stable linoleic acid oxidation product 13-HODE + 9-HODE and was related to increases in F2-isoprostanes, linoleate, and fatty acids in the linoleate conversion pathway. These data support the use of 13-HODE + 9-HODE as an oxidative stress biomarker in acute exercise investigations.
生物活性氧化亚油酸代谢物（OXLAMs）包括 13-和 9-羟基十八碳二烯酸（13-HODE + 9-HODE），与氧化应激、炎症和许多病理及生理状态有关。本研究的目的是测量 75 公里骑行后血浆 13-HODE + 9-HODE 的变化，并通过代谢组学方法鉴定潜在的亚麻酸代谢和氧化应激（F2-异前列腺素）及炎症（细胞因子）的建立生物标志物。经过训练的男性自行车运动员（N = 19，年龄 38.0 ± 1.6 岁，最大瓦特 304 ± 10.5）在他们自己的自行车上使用电磁制动自行车测力计（2.71 ± 0.07 h）进行 75 公里计时赛。运动前、运动后即刻、1.5 小时和 21 小时采集血样，使用气相色谱-质谱联用仪（GC-MS）和液相色谱-质谱联用仪（LC-MS）进行的全局代谢组学程序分析血浆细胞因子（IL-6、IL-8、IL-10、肿瘤坏死因子-α、单核细胞趋化蛋白-1、粒细胞集落刺激因子）、F2-异前列腺素和代谢物的变化。13-HODE + 9-HODE 在运动后即刻和 1.5 小时增加了 3.1 倍和 1.7 倍（均 P < 0.001），并在 21 小时后恢复到运动前水平。75 公里骑行后血浆 13-HODE + 9-HODE 水平与血浆细胞因子的增加无显著相关性，但与运动后 F2-异前列腺素（r = 0.75，P < 0.001）、亚麻酸（r = 0.54，P = 0.016）、花生四烯酸（r = 0.77，P < 0.001）、12,13-二羟基-9Z-十八碳烯酸（12,13-DiHOME）（r = 0.60，P = 0.006）、二氢亚麻酸（r = 0.57，P = 0.011）和肾上腺素（r = 0.56，P = 0.013）呈正相关。这些发现表明，长时间剧烈运动导致稳定的亚油酸氧化产物 13-HODE + 9-HODE 短暂增加了 3.1 倍，与 F2-异前列腺素、亚麻酸和亚麻酸转化途径中的脂肪酸增加有关。这些数据支持在急性运动研究中使用 13-HODE + 9-HODE 作为氧化应激生物标志物。
