定期随访的 HIV 患者作为流式细胞术检测淋巴细胞亚群的内部质量控制的价值。
Value of HIV patients with regular follow-up as in-house internal controls of flow cytometry measurement of lymphocyte subsets.
机构信息
Immunology Laboratory, CHU de Nancy & Lorraine Université, Vandoeuvre les Nancy, France.
出版信息
Cytometry B Clin Cytom. 2013 Sep-Oct;84(5):324-6. doi: 10.1002/cyto.b.21112. Epub 2013 Aug 8.
BACKGROUND
Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow-up of known patients may provide a robust in-house control of cell subsets evaluation.
METHODS
Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells, and CD4+ absolute counts (ACs).
RESULTS
Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08, and 0.18 for CD3%, CD4%, CD8%, and CD4 ACs, respectively.
DISCUSSION
In-house follow-up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. © 2013 International Clinical Cytometry Society.
背景
流式细胞术的质量评估不能遵循适用于化学分析物测量的相同规则。然而，对已知患者的定期随访可能为细胞亚群评估提供稳健的内部对照。
方法
对多年来 32 名 HIV 患者的连续血液样本进行评估，结果显示稳定性良好，对其进行回顾性评估以确定 CD3+、CD4+、CD8+细胞百分比和 CD4+绝对计数 (AC) 的变异系数。
结果
整个队列的平均相对标准偏差分别为 0.04、0.14、0.08 和 0.18，用于 CD3%、CD4%、CD8%和 CD4 ACs。
讨论
定期检查的依从性患者的内部随访是对传统且昂贵的重复性和再现性研究的替代方法，可用于验证常规流式细胞术。© 2013 年国际临床细胞化学学会。
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