State Key Laboratory of Natural and Biomimetic Drugs (Peking University), Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University , Beijing , China .
Pharm Biol. 2013 Nov;51(11):1384-92. doi: 10.3109/13880209.2013.793719. Epub 2013 Aug 12.
Coptidis Rhizoma-Euodiae Fructus couple (CEC) is a classic traditional Chinese medicine preparation consisting of Coptidis Rhizoma and Euodiae Fructus at the ratio of 6:1, and used to treat gastro-intestinal disorders. Alkaloids are the main bioactive component. This research provides comprehensive analysis information for the quality control of CEC.
To develop a high-performance liquid chromatography-diode array detection fingerprint for chemical composition characteristics of CEC and its products.
The samples were separated with a Gemini C18 column by using gradient elution with water-formic acid (100:0.03) and acetonitrile as mobile phase. Flow rate was 1.0 mL/min and detection wavelength was 250 nm. Similarity analysis and principal component analysis (PCA) were employed to evaluate quality consistencies of analytes. Mean chromatograms and correlation coefficients of analytes were calculated by the software "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine".
Fingerprint chromatogram comparison determined 20 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.988. Consistent results were obtained to show that CEC and its related samples could be successfully divided into three groups. Contribution plots generated by PCA were performed to interpret differences among the sample groups while peaks which significantly contributed to classification were identified. Seven bioactive constituents in the samples were verified by quantitative analysis.
The chromatographic fingerprint with similarity evaluation and PCA assay combined with quantification of seven compounds could be utilized as a quality control method for the herbal couple.
黄连-吴茱萸药对（CEC）由黄连和吴茱萸以 6:1 的比例组成，是一种经典的中药制剂，用于治疗胃肠疾病。生物碱是主要的生物活性成分。本研究为 CEC 及其制剂的质量控制提供了全面的分析信息。
建立一种高效液相色谱-二极管阵列检测法用于分析 CEC 及其制剂的化学成分特征指纹图谱。
采用 Gemini C18 柱，以水-甲酸（100:0.03）和乙腈为流动相进行梯度洗脱，流速为 1.0ml/min，检测波长为 250nm。采用相似度分析和主成分分析（PCA）对供试品的质量一致性进行评价。采用“中药色谱指纹图谱相似度评价系统”软件计算各成分的平均色谱图和相关系数。
指纹图谱比较确定了 20 个代表性的共有指纹峰，指纹图谱相似度均大于 0.988。结果一致，表明 CEC 及其相关样品可成功分为三组。PCA 生成的贡献图用于解释样品组之间的差异，同时确定了对分类有显著贡献的峰。对样品中的 7 种生物活性成分进行了定量分析。
采用相似度评价和 PCA 分析结合定量分析 7 种化合物的色谱指纹图谱可作为该草药对的质量控制方法。
