Pepeu G, Giovannelli L
Dipartimento di Farmacologia Preclinica e Clinica, Università degli Studi, Florence, Italy.
Ann Ist Super Sanita. 1990;26(1):49-53.
Cytosolic Ca2+ concentrations [( Ca2+]i) were measured in synaptosomes prepared from the cerebral cortex of 3, 16 and 24 month-old male Charles River Wistar rats. Electron microscopy examination demonstrated no morphological differences between the synaptosomes prepared from 3 and 24 month-old rats. Age did not modify [Ca2+]i, as measured by the QUIN 2 technique, both at rest and immediately after depolarization with 50 mM K+. The Ca2+ load following depolarization was cleared in about 13 min in the 3 month-old rats. The rate of clearance was significantly slower both in the (p less than 0.01) and in the 24 month-old (p less than 0.0001). A prolonged calcium influx may be responsible for the slower clearance of Ca2+ load in aged rats.
在3个月、16个月和24个月大的雄性查尔斯河Wistar大鼠大脑皮层制备的突触体中测量胞质Ca2+浓度[（Ca2+]i）。电子显微镜检查显示，3个月和24个月大的大鼠制备的突触体之间没有形态学差异。通过QUIN 2技术测量，年龄在静息状态和用50 mM K+去极化后均未改变[Ca2+]i。3个月大的大鼠去极化后的Ca2+负荷在约13分钟内清除。清除率在16个月大的大鼠中显著减慢（p小于0.01），在24个月大的大鼠中更是显著减慢（p小于0.0001）。钙内流延长可能是老年大鼠Ca2+负荷清除较慢的原因。
