Baylor Institute for Immunology Research, Baylor Research Institute, 3434 Live Oak, Dallas, TX 75204, USA.
Cell Immunol. 2013 May-Jun;283(1-2):81-90. doi: 10.1016/j.cellimm.2013.06.004. Epub 2013 Jun 19.
The aim of this study is to show the effect of donor-specific transfusion (DST) in inducing immunological tolerance mediated by regulatory T cells (Treg) and indoleamine 2,3-dioxygenase (IDO). Skin grafts from H2(d) Balb/c were transplanted into H2(k) C3H/He 7days after the infusion of donor splenocytes, isolated each immune cell populations. Graft survival prolonged in recipients who received splenocytes, MHC class II(+) CD90(-) cells and CD3(-)CD19(-) cells (p<0.001, p<0.05 and p<0.01, respectively). CD11b(+) cell infusion resulted in prolongation of graft survival when compared to CD11c(+) cell infusion (p<0.01). Foxp3(+)CD4(+)CD25(+) T cells were increased after the transplant in recipients infused with CD11b(+) cells (p<0.05). The mixed lymphocyte reaction showed donor-specificity (p<0.001). High IDO expression was observed in CD11b(+) cell infusion group. Graft survival with DST using IDO antagonist (1MT) were not prolonged. In conclusion, DST allows induction of donor-specific tolerance which involves Foxp3(+)CD4(+)CD25(+) T cells and IDO expression.
本研究旨在展示供者特异性输血（DST）通过调节性 T 细胞（Treg）和吲哚胺 2,3-双加氧酶（IDO）诱导免疫耐受的效果。在输注供者脾细胞 7 天后，将 H2(d)Balb/c 皮肤移植物移植到 H2(k)C3H/He 中，分离每种免疫细胞群。接受脾细胞、MHC Ⅱ类（+）CD90（-）细胞和 CD3（-）CD19（-）细胞输注的受者移植物存活时间延长（p<0.001、p<0.05 和 p<0.01）。与 CD11c（+）细胞输注相比，CD11b（+）细胞输注导致移植物存活时间延长（p<0.01）。与未输注 CD11b（+）细胞的受者相比，输注 CD11b（+）细胞后 Foxp3（+）CD4（+）CD25（+）T 细胞增加（p<0.05）。混合淋巴细胞反应显示供者特异性（p<0.001）。CD11b（+）细胞输注组中观察到 IDO 表达增加。使用 IDO 拮抗剂（1MT）进行 DST 并未延长移植物存活时间。总之，DST 可诱导涉及 Foxp3（+）CD4（+）CD25（+）T 细胞和 IDO 表达的供者特异性耐受。
