Division of Genetics, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.
Cancer Lett. 2013 Nov 28;341(1):105-10. doi: 10.1016/j.canlet.2013.08.001. Epub 2013 Aug 7.
With an increase of neoadjuvant therapy, biopsy samples have become imperative for cancer research; however, what kind of difference between surgical and endoscopic biopsy samples in gene expression profiles was still unclear. Recently, we reported artificially induced epithelial-mesenchymal transition (aiEMT) in the surgical samples by comparison between gene expression profiles of both samples of the esophagus. This was also found in mouse epithelium under an ischemic condition for 4h. This study will evoke underestimation of the prognostic evaluation power of EMT related markers in past cancer research and prevalence of biopsy samples for in vivo expression profiling with low biases.
随着新辅助治疗的增加，活检样本对于癌症研究变得至关重要；然而，手术和内镜活检样本在基因表达谱上的差异仍不清楚。最近，我们通过比较食管的两种样本的基因表达谱，报告了在手术样本中人为诱导的上皮-间充质转化（aiEMT）。在 4 小时的缺血条件下，在小鼠上皮中也发现了这种情况。这项研究将低估 EMT 相关标志物在过去癌症研究中的预后评估能力，并导致在体内表达谱中使用低偏倚的活检样本的普遍性。
