Department of Ichthyology and Aquatic Environment, University of Thessaly, Volos 38446, Greece.
Anaerobe. 2013 Oct;23:9-11. doi: 10.1016/j.anaerobe.2013.07.010. Epub 2013 Aug 6.
By comparing 16S rDNA cloning and 454 pyrosequencing in the Nephrops norvegicus midgut, several common bacterial OTUs were detected. However, when only one method is to be selected, it needs to be considered whether the revealing of rare OTUs or their accurate phylogenetic relationships is mostly preferred.
通过比较欧洲龙虾中肠的 16S rDNA 克隆和 454 焦磷酸测序,检测到了几个常见的细菌 OTUs。然而,当只能选择一种方法时,需要考虑是更倾向于揭示稀有 OTUs 还是更倾向于它们准确的系统发育关系。