Biozentrum, University of Basel, Switzerland; Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, Ramistrasse 100, CH-8091 Zurich, Switzerland.
Mol Immunol. 2013 Dec;56(4):683-92. doi: 10.1016/j.molimm.2013.05.003. Epub 2013 Aug 8.
Coronin 1 is a member of the evolutionary conserved WD repeat protein family and is highly expressed in hematopoietic cells. Coronin 1 is essential for Ca(2+) mobilization upon T cell receptor (TCR) stimulation providing a pro-survival signal for naïve peripheral T cells. Both in mouse and in human, coronin 1 deficiency is associated with severe T cell lymphopenia. In this work, we have analyzed antiviral T cell-mediated immunity in the presence and absence of coronin 1 in vivo after infection with lymphocytic choriomenigitis virus (LCMV) and vesicular stomatitis virus (VSV) in mice. Despite low peripheral T cell numbers we found that LCMV-specific CD8(+) T cell responses were normal in the absence of coronin 1 and kinetics of LCMV-clearance were similar compared to wild type mice. In contrast, CD4(+) T cell responses were profoundly decreased after LCMV- and VSV-infection. We propose that coronin 1 plays a differential role in CD8(+) versus CD4(+) T cell responses and activation.
冠状蛋白 1 是进化上保守的 WD 重复蛋白家族的成员，在造血细胞中高度表达。冠状蛋白 1 在 T 细胞受体 (TCR) 刺激后钙 (Ca2+) 动员中是必需的，为幼稚外周 T 细胞提供了一个生存信号。在小鼠和人类中，冠状蛋白 1 的缺乏都与严重的 T 细胞淋巴细胞减少症有关。在这项工作中，我们分析了在感染淋巴细胞性脉络丛脑膜炎病毒 (LCMV)和水疱性口炎病毒 (VSV)后体内存在和不存在冠状蛋白 1 时的抗病毒 T 细胞介导的免疫。尽管外周 T 细胞数量较低，但我们发现缺乏冠状蛋白 1 时 LCMV 特异性 CD8(+) T 细胞反应正常，与野生型小鼠相比，LCMV 清除的动力学相似。相比之下，在 LCMV 和 VSV 感染后，CD4(+) T 细胞反应明显减少。我们提出，冠状蛋白 1 在 CD8(+)与 CD4(+) T 细胞反应和激活中发挥不同的作用。
