一种基于局域表面等离子体共振生物传感的用于测定沙丁胺醇的无标记免疫传感器。
A label-free immunosensor for determination of salbutamol based on localized surface plasmon resonance biosensing.
作者信息
Yan Ziren, Hu Tingting, Guo Wenying, Deng Anping, Di Junwei
机构信息
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, 215123, Jiangsu, People's Republic of China.
出版信息
Bioprocess Biosyst Eng. 2014 Apr;37(4):651-7. doi: 10.1007/s00449-013-1034-z. Epub 2013 Aug 10.
We developed a localized surface plasmon resonance (LSPR)-based label-free optical biosensor for detection of salbutamol (Sal). Hollow gold nanoparticles (HGNs) which deposited on transparent indium tin oxide (ITO) film coated glass was used to sensing platform. Antibody against Sal was immobilized on HGN surface to recognize the target Sal molecules. Thus, the change of LSPR peak was proportional to the concentration of Sal in the solution. The experimental results demonstrated that the LSPR immunosensor possessed a good sensitivity and a high selectivity for Sal. The detection range for Sal was from 0.05 to 0.8 μg/mL with a correlation coefficient of 0.996. The biosensor was applied for the detection for Sal in spiked animal feed and pork liver samples, and the recoveries were in the range of 97-105 %. Therefore, it is expected that this approach may offer a new method in designing label-free LSPR immunosensor for detection of small molecules.
我们开发了一种基于局域表面等离子体共振（LSPR）的无标记光学生物传感器，用于检测沙丁胺醇（Sal）。沉积在涂有透明氧化铟锡（ITO）薄膜的玻璃上的中空金纳米颗粒（HGNs）被用作传感平台。抗Sal抗体固定在HGN表面，以识别目标Sal分子。因此，LSPR峰的变化与溶液中Sal的浓度成正比。实验结果表明，LSPR免疫传感器对Sal具有良好的灵敏度和高选择性。Sal的检测范围为0.05至0.8μg/mL，相关系数为0.996。该生物传感器用于加标动物饲料和猪肝样品中Sal的检测，回收率在97%-105%范围内。因此，预计这种方法可能为设计用于检测小分子的无标记LSPR免疫传感器提供一种新方法。
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