Clozza Emanuele, Obrecht Marcel, Dard Michel, Coelho Paulo G, Dahlin Christer, Engebretson Steven P
Department of Periodontology and Ashman Department of Implant Dentistry, New York University College of Dentistry, 345 East 24th Street, Suite 3W, New York, NY, 10010, USA.
Institut Straumann AG, 4002, Basel, Switzerland.
Clin Oral Investig. 2014 May;18(4):1245-1250. doi: 10.1007/s00784-013-1081-5. Epub 2013 Aug 10.
The aim of the study was to introduce a novel three-dimensional (3D) method to quantify the relative amount of different tissue components in bone substitute-treated defects by means of integration of confocal laser imaging into micro-computed tomography (μCT) analysis.
One standardized semisaddle intraosseous defect was prepared in the mandibles of six minipigs and scanned by an optical scanner to capture the surface of the fresh defect in a 3D manner. Subsequently, all the defects were filled with a biphasic calcium phosphate material. The animals were divided into two groups of three animals each, which were allowed to heal for 3 and 8 weeks, respectively. μCT analysis followed the two healing periods and was performed on all defect locations. The data from optical scanning and μCT were used for three-dimensional evaluation of bone formation, nonmineralized tissue ratio, and graft degradation. The integration of confocal laser scanning into μCT analysis through a superimposition imaging procedure was conducted using the software Amira (Mercury Computer Systems, Chelmsford, MA, USA).
The feasibility of combining the confocal imaging into μCT data with regard to obtaining accurate 3D quantification was demonstrated. The amount of tissue components was identified and quantified in all the investigated samples. Quantitative analysis demonstrated that a significant increase in the amount of bone filling the defect was observed in vivo (p < 0.02) while a significant decrease in the amount of nonmineralized tissue occurred (p < 0.04). No difference in the amount of residual grafting material was detected between 3 and 8 weeks in vivo (p > 0.38).
The combination of confocal imaging and micro-computed tomography techniques allows for analysis of different tissue types over time in vivo. This method has revealed to be a feasible alternative to current bone regeneration quantification methods.
Assessment of bone formation in a large animal model is a key step in assessing the performance of new bone substitute materials. Reliable and accurate methods are needed for the analysis of the regenerative potential of new materials.
本研究的目的是引入一种新颖的三维（3D）方法，通过将共聚焦激光成像整合到微计算机断层扫描（μCT）分析中，来量化骨替代物治疗缺损中不同组织成分的相对含量。
在六只小型猪的下颌骨中制备一个标准化的半鞍状骨内缺损，并用光学扫描仪进行扫描，以三维方式获取新鲜缺损的表面。随后，所有缺损均用双相磷酸钙材料填充。将动物分为两组，每组三只，分别让其愈合3周和8周。在两个愈合期后对所有缺损部位进行μCT分析。光学扫描和μCT的数据用于骨形成、非矿化组织比例和移植物降解的三维评估。使用Amira软件（美国马萨诸塞州切尔姆斯福德市水星计算机系统公司）通过叠加成像程序将共聚焦激光扫描整合到μCT分析中。
证明了将共聚焦成像与μCT数据相结合以获得准确三维定量的可行性。在所有研究样本中识别并量化了组织成分的含量。定量分析表明，在体内观察到填充缺损的骨量显著增加（p < 0.02），而非矿化组织的量显著减少（p < 0.04）。在体内3周和8周之间未检测到残留移植材料量的差异（p > 0.38）。
共聚焦成像和微计算机断层扫描技术的结合允许在体内随时间分析不同组织类型。该方法已被证明是当前骨再生定量方法的一种可行替代方法。
在大型动物模型中评估骨形成是评估新型骨替代材料性能的关键步骤。需要可靠且准确的方法来分析新材料的再生潜力。
