伊朗阿尔达比勒省MTHFR基因C677T多态性与胃癌易感性降低的关系

Decrease in gastric cancer susceptibility by MTHFR C677T polymorphism in Ardabil Province, Iran.

作者信息

Hosseini-Asl S Saied, Pourfarzi Farhad, Barzegar Abazar, Mazani Mohammad, Farahmand Nima, Niasti Elham, Yazdanbod Abbas, Didevar Reza, Akhavan Homa, Malekzadeh Reza

机构信息

Ardabil University of Medical Sciences, Gastrointestinal Research Center, Ardabil, Islamic Republic of Iran.

出版信息

Turk J Gastroenterol. 2013;24(2):117-21. doi: 10.4318/tjg.2013.0572.

DOI:10.4318/tjg.2013.0572

PMID:23934457

Abstract

BACKGROUND/AIMS: Gastric cancer, as the fourth most frequent malignancy worldwide, has the highest rate among cancer-related disorders in Ardabil province, located in North-West Iran. Methylene tetrahydrofolate reductase is one of the cancer susceptibility genes with considerable polymorphisms. Methylene tetrahydrofolate reductase C677T leads to a decrease of about 30% in its product activity and is reported to have an effect on cancer susceptibility.

MATERIALS AND METHODS

Methylene tetrahydrofolate reductase C677T genotyping was carried out by polymerase chain reaction-restriction fragment length polymorphism on peripheral blood DNA from 76 gastric cancer patients and 91 healthy controls. The statistical significance was calculated by logistic regression analysis.

RESULTS

The mean age was 64.2±11.1 and 62.1±9.8 years for cases and controls, respectively. Among tumors, 35.5% were diffuse type and others were intestinal. The frequency of genotypes was 61.8%, 32.9%, and 5.3% among cases and 45.1%, 50.6%, and 4.4% among controls for Ala/Ala, Ala/Val, and Val/Val, respectively. CT heterozygotes had lower susceptibility to gastric cancer (p=0.02). This relationship of significance was detected considering gender and age.

CONCLUSIONS

It was found that T allele has a protective association with age in the Ardabil province.

摘要

背景/目的：胃癌是全球第四大常见恶性肿瘤，在伊朗西北部的阿尔达比勒省，其在癌症相关疾病中的发病率最高。亚甲基四氢叶酸还原酶是一种具有相当多态性的癌症易感基因。亚甲基四氢叶酸还原酶C677T导致其产物活性降低约30%，据报道对癌症易感性有影响。

材料与方法

采用聚合酶链反应-限制性片段长度多态性方法，对76例胃癌患者和91例健康对照者的外周血DNA进行亚甲基四氢叶酸还原酶C677T基因分型。通过逻辑回归分析计算统计学意义。

结果

病例组和对照组的平均年龄分别为64.2±11.1岁和62.1±9.8岁。在肿瘤中，35.5%为弥漫型，其他为肠型。病例组中Ala/Ala、Ala/Val和Val/Val基因型的频率分别为61.8%、32.9%和5.3%，对照组中分别为45.1%、50.6%和4.4%。CT杂合子对胃癌的易感性较低（p=0.02）。考虑性别和年龄后检测到这种显著关系。