酶在异功能乙醛酸载体上的定向共价固定化。

Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports.

作者信息

Mateo Cesar, Fernandez-Lorente Gloria, Rocha-Martin Javier, Bolivar Juan M, Guisan Jose M

机构信息

Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain.

出版信息

Methods Mol Biol. 2013;1051:73-88. doi: 10.1007/978-1-62703-550-7_6.

DOI:10.1007/978-1-62703-550-7_6

PMID:23934799

Abstract

Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contained the maximal concentration of glyoxyl groups to promote maximization of covalent immobilization and groups' capability to adsorb proteins by various mechanisms (e.g., ionic exchange, metal-chelate formation). Immobilization on various supports makes it possible to orientate and rigidify an enzyme in various regions of its surface. The use of different heterofunctional supports allowed for obtaining catalysts with different activity, stability, and selectivity properties.

摘要

制备了新型异功能乙醛酸琼脂糖载体。这些载体含有最大浓度的乙醛酸基团，以促进共价固定的最大化以及基团通过各种机制（例如离子交换、金属螯合物形成）吸附蛋白质的能力。固定在各种载体上能够使酶在其表面的不同区域进行定向和刚性化。使用不同的异功能载体能够获得具有不同活性、稳定性和选择性的催化剂。