将皱褶假丝酵母脂肪酶固定在超顺磁性Fe3O4纳米颗粒上用于低水介质中的生物催化。
Immobilization of Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles for biocatalysis in low-water media.
作者信息
Mukherjee Joyeeta, Solanki Kusum, Gupta Munishwar Nath
机构信息
Department of Chemistry, Indian Institute of Technology Delhi, New Delhi, India.
出版信息
Methods Mol Biol. 2013;1051:117-27. doi: 10.1007/978-1-62703-550-7_8.
A simple immobilization method for Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles is described. The Fe3O4 nanoparticles were coated with PEI and Candida rugosa lipase was adsorbed on these particles via electrostatic interactions. The immobilization resulted in marginal simultaneous purification. However, the immobilized preparation showed 110× higher transesterification activity in low-water media. It was also efficient in kinetic resolution of (±)-1-phenylethanol with eep of 99 % and E = 412 within 24 h.
描述了一种将皱褶假丝酵母脂肪酶固定在超顺磁性Fe3O4纳米颗粒上的简单固定化方法。Fe3O4纳米颗粒用聚乙烯亚胺（PEI）包覆，皱褶假丝酵母脂肪酶通过静电相互作用吸附在这些颗粒上。固定化导致了一定程度的同步纯化。然而，固定化制剂在低水介质中的酯交换活性提高了110倍。它在（±）-1-苯乙醇的动力学拆分中也很有效，在24小时内对映体过量值（eep）为99%，对映体选择性（E）为412。
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