Roy Ipsita, Mukherjee Joyeeta, Gupta Munishwar Nath
Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Punjab, India.
Methods Mol Biol. 2013;1051:275-84. doi: 10.1007/978-1-62703-550-7_18.
Simple precipitation of enzymes has shown impressive catalytic efficiencies in organic solvents. In asmuch as these can be recovered after the reaction, these can be viewed as immobilized preparations just like more extensively used cross-linked enzyme aggregates (CLEAs). This chapter describes three protocols which use these enzyme precipitated and rinsed with propanol/some other appropriate organic solvent. The first two protocols show their applications in ionic liquids for a transesterification reaction and a kinetic resolution. The third protocol presumably incorporates an "imprinting" effect so that the precipitates are now able to efficiently catalyze transesterification of tributyrin with tertiary alcohols.
酶的简单沉淀在有机溶剂中已显示出令人印象深刻的催化效率。由于这些酶在反应后可以回收，因此它们可以被视为固定化制剂，就像使用更广泛的交联酶聚集体（CLEAs）一样。本章介绍了三种使用用丙醇/其他合适的有机溶剂沉淀并冲洗过的酶的方法。前两种方法展示了它们在离子液体中用于酯交换反应和动力学拆分的应用。第三种方法可能包含一种“印记”效应，使得沉淀物现在能够有效地催化三丁酸甘油酯与叔醇的酯交换反应。
