靶向DNA测序：使用混合寡脱氧核苷酸探针作为引物对DNA进行测序，从而快速鉴定DNA克隆。
Targeted DNA sequencing: rapid identification of DNA clones by sequencing DNA using mixed oligodeoxynucleotide probes as primers.
作者信息
Javed A A, Joshi S
机构信息
Department of Obstetrics & Gynecology, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
Biotechniques. 1990 Jul;9(1):28-32.
PMID:2393569
Abstract
A rapid identification method involving targeted DNA sequencing of genomic or cDNA clones using mixed (degenerate) probes as primers is described. The strategy involves the use of the same mixed probes for sequencing the clone of interest as they are used for screening the DNA libraries. Probes containing up to 512 mixes do not interfere in priming and yield completely faithful replication of the template DNA.
摘要
本文描述了一种快速鉴定方法，该方法涉及使用混合（简并）探针作为引物对基因组或cDNA克隆进行靶向DNA测序。该策略涉及使用与筛选DNA文库相同的混合探针来对感兴趣的克隆进行测序。包含多达512种混合物的探针不会干扰引物作用，并能完全忠实复制模板DNA。
Zotero 插件