Tongboonchoo Chutaporn, Tungtrongchitr Anchalee, Phonrat Benjaluck, Preutthipan Sangchai, Tungtrongchitr Rungsunn
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University Bangkok, Thailand.
J Med Assoc Thai. 2013 Feb;96(2):133-9.
Osteoporosis and osteopenia is rising with the increase in numbers of postmenopausal women. Methylenetetrahydrofolate reductase (MTHFR), a homocysteine catabolizing enzyme, is involved in the regulation of bone mineral density (BMD). The association between MTHFR C677T polymorphism with osteoporosis in postmenopausal Thai women is hitherto unclear.
To investigate the association between MTHFR C677T and BMD in postmenopausal Thai women.
The study subjects consisted of 346 postmenopausal Thai women volunteers. Standard dual-energy X-ray absorptiometry (DXA) was used for measurement of BMD T-score. Restriction fragment length polymorphism (RFLP) analysis was used for measurement of MTHFR C677T polymorphism.
In the evaluation of 346 postmenopausal Thai women heterozygous (CT) genotype had a risk of osteopenia than normal control (odds ratio (OR) = 5.66, p < 0.001). BMD T-scores at each bone position revealed that heterozygous (CT) genotype had increased risk of osteopenic bones than normal controls at lumbar spines 1, 2, and 4 (OR = 2.48, p < 0.001, OR = 1.98, p = 0.008 and OR = 1.83, p = 0.016 respectively), ward's triangle (OR = 2.08, p = 0.008), and head of radius (OR = 2.95, p = 0.008).
These results indicate the possibility of using MTHFR C677T polymorphism to identify postmenopausal Thai women at high risk of osteopenia.
随着绝经后女性数量的增加，骨质疏松症和骨质减少的情况也在上升。亚甲基四氢叶酸还原酶（MTHFR）是一种参与同型半胱氨酸分解代谢的酶，它参与骨矿物质密度（BMD）的调节。迄今为止，泰国绝经后女性中MTHFR C677T基因多态性与骨质疏松症之间的关联尚不清楚。
研究泰国绝经后女性中MTHFR C677T与骨矿物质密度之间的关联。
研究对象包括346名泰国绝经后女性志愿者。采用标准双能X线吸收法（DXA）测量骨矿物质密度T值。采用限制性片段长度多态性（RFLP）分析来检测MTHFR C677T基因多态性。
在对346名泰国绝经后女性的评估中，杂合子（CT）基因型患骨质减少症的风险高于正常对照组（比值比（OR）=5.66，p<0.001）。每个骨位的骨矿物质密度T值显示，杂合子（CT）基因型在腰椎1、2和4处（OR分别为2.48，p<0.001；OR为1.98，p=0.008；OR为1.83，p=0.016）、沃德三角区（OR=2.08，p=0.008）和桡骨小头处（OR=2.95，p=0.008）患骨质减少性骨病的风险高于正常对照组。
这些结果表明，利用MTHFR C677T基因多态性来识别有骨质减少症高风险的泰国绝经后女性具有可能性。
