Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664, USA.
Anal Biochem. 2013 Nov 15;442(2):146-8. doi: 10.1016/j.ab.2013.08.003. Epub 2013 Aug 11.
A simple, inexpensive microdistillation device is described for capturing methanol or formaldehyde as end products of biochemical reactions or in environmental samples. We demonstrate that the microdistillation protocol, coupled with the use of alcohol oxidase and the formaldehyde-sensitive reagent Purpald (4-amino-3-hydrazino-5-mercapto-1,2,4-triazole), serves as a quick and inexpensive alternative to chromatographic and mass spectrometer analyses for determining if formaldehyde or methanol is a product of reactions that contain substances that interfere with the Purpald reaction. These techniques were used to affirm formaldehyde as the end product of the dicamba monooxygenase-catalyzed O-demethylation of the herbicide dicamba (2-methoxy-3,6-dichlorobenzoic acid).
介绍了一种简单、廉价的微蒸馏装置，用于捕获生物化学反应或环境样品中的甲醇或甲醛作为终产物。我们证明，微蒸馏方案结合使用酒精氧化酶和甲醛敏感试剂 Purpald（4-氨基-3-肼基-5-巯基-1,2,4-三唑），可作为一种快速且廉价的替代色谱和质谱分析方法，用于确定甲醛或甲醇是否是含有干扰 Purpald 反应物质的反应的产物。这些技术被用于确认甲醛是除草剂麦草畏（2-甲氧基-3,6-二氯苯甲酸）单加氧酶催化的 O-去甲基化反应的终产物。
