肾干细胞对人肾小管上皮细胞损伤修复的影响

[Effects of kidney stem cells on injure-recovery of human renal tubular epithelial cells].

作者信息

Wen Jing, Cheng Qing-li, Ma Qiang, Qi Yun, Zhao Jia-hui, Du Jing, Wang Xiao-dan, Liu Sheng, Li Mei Hua, Zhang Xiao Ying

机构信息

Department of Geriatric Nephrology, Institute of Gerontology, Chinese PLA General Hospital, Beijing 100853, China.

出版信息

Beijing Da Xue Xue Bao Yi Xue Ban. 2013 Aug 18;45(4):619-24.

PMID:23939175

Abstract

OBJECTIVE

To investigate the protective effects and the mechanism of kidney stem cells (KSC) on the injured tubular epithelial cells.

METHODS

KSC were isolated from rat renal papilla. The human kidney epithelial cells (HKC) injury model was induced with 0.1 μmol/L antimycin A for 30 minutes. The injured HKC were co-cultured with KSC or supernatant of cultured KSC. The apoptosis of HKC were detected by flow cytometry. The changes of ATP in the HKC and the level of malondialdehyde (MDA), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) in the supernatant of cultured HKC were detected after being co-cultured with KSC.

RESULTS

The study of the co-culture showed that KSC was less capable to migrate through the micropores of Transwell compared with bone marrow stem cells. However, after being co-cultured with the KSC conditional supernatant, ATP content of injured HKC, total SOD value in the supernatant of injured HKC were increased, and the MDA and LDH in the supernatant of injured HKC decreased.

CONCLUSION

KSC have protected effects and participate in the repair of injured HKC.

摘要

目的

探讨肾干细胞（KSC）对损伤的肾小管上皮细胞的保护作用及其机制。

方法

从大鼠肾乳头分离出KSC。用0.1μmol/L抗霉素A诱导人肾上皮细胞（HKC）损伤模型30分钟。将损伤的HKC与KSC或培养的KSC上清液共培养。通过流式细胞术检测HKC的凋亡情况。与KSC共培养后，检测HKC中ATP的变化以及培养的HKC上清液中丙二醛（MDA）、超氧化物歧化酶（SOD）和乳酸脱氢酶（LDH）的水平。