Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.
Biochimie. 2013 Nov;95(11):2091-6. doi: 10.1016/j.biochi.2013.07.025. Epub 2013 Aug 11.
Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards β-brominated alkanes. Moreover, we identified other putative haloalkane dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.
卤代烷烃脱卤酶是一类能够水解卤代化合物中碳卤键的细菌酶。本文首次报道了来自紫海胆的具有生物化学特征的非微生物卤代烷烃脱卤酶 DspA。该酶优先作用于末端溴代烃，且对β-溴代烷烃具有对映选择性。此外，我们还鉴定了其他可能具有真核起源的卤代烷烃脱卤酶，这些酶是未来发现具有新型催化特性的脱卤酶的潜在靶标。
