Advanced Photonics Center, Southeast University, 210096, China.
Biosens Bioelectron. 2014 Jan 15;51:82-9. doi: 10.1016/j.bios.2013.07.034. Epub 2013 Jul 26.
We developed a dual-mode detection method for tumor cell specific targeting and intracellular delivery of the chemotherapeutic agent Doxorubicin (DOX) using folic acid functionalized mesoporous silica nanoparticles (FA-MSNs) as carrier systems. In this method, label free surface enhanced Raman scattering (SERS) spectra were utilized to monitor the dynamic release of DOX inside tumor cells in combination with fluorescence images. To investigate the targeting delivery performance of the carrier system, both normal cells (MRC-5) and tumor cells (HeLa) were used as the model cells. The real-time release of DOX from FA-MSNs inside MRC-5 and HeLa cells was monitored. As demonstrated by both fluorescence and SERS results, the DOX loaded FA-MSNs can actively target FA receptor overexpressed tumor cells. Moreover, the releasing behavior of DOX from FA-MSNs in tumor and in normal cells was quantitatively analyzed. Compared with the traditional sole fluorescence or SERS method, this dual-mode detection is more powerful and more accurate, which should have a potential application in drug tracking in living cells.
我们开发了一种双模式检测方法，用于肿瘤细胞特异性靶向和细胞内递送达比柔比星（DOX）等化疗药物，方法是使用叶酸功能化介孔硅纳米粒子（FA-MSNs）作为载体系统。在该方法中，我们利用无标记表面增强拉曼散射（SERS）光谱结合荧光图像来监测 DOX 在肿瘤细胞内的动态释放。为了研究载体系统的靶向递药性能，我们以正常细胞（MRC-5）和肿瘤细胞（HeLa）作为模型细胞。实时监测了 DOX 从 FA-MSNs 向 MRC-5 和 HeLa 细胞内的释放。荧光和 SERS 结果均表明，载 DOX 的 FA-MSNs 可以主动靶向过表达 FA 受体的肿瘤细胞。此外，我们还定量分析了 DOX 从 FA-MSNs 在肿瘤细胞和正常细胞中的释放行为。与传统的单一荧光或 SERS 方法相比，这种双模式检测方法更强大、更准确，有望在活细胞中的药物追踪中得到应用。
