Wu Hai-Yan, Chu Hai-Rong, Li Hong, Tang Ke-Xin, Jing Xu, Sun Jin-Long, Yin Qing-Ling, Cheng Min
Department of Anesthesiology, Weifang Medical University, Weifang 261053, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2013 May;29(3):199-202.
To investigate the effects of endothelia progenitor cells conditioned medium (EPC-CM) on the migration, adhesion and proliferation of vascular smooth muscle cells (VSMCs).
Mononuclear cells were isolated from rat bone marrow by density gradient centrifugation,plated on dishes precoated with 5% fibronectin, and then cultured with complete M199 medium (including 15% fetal calf serum, 10 microg/L VEGF and 5 microg/L bFGF). EPC-CM was collected and used to incubate VSMCs isolated from rat arteriae aorta. After 24 h, VSMCs proliferation, adhesion and migration were assayed with CCK-8, adhesion test and modified Boyden chamber assay, respectively.
The proliferation, adhesion and migration of VSMCs were obviously decreased when the cells were cultured with EPC-CM.
EPC-CM could inhibit VSMC functions, which would be one of the mechanisms against atherosclerosis by EPCs.
