Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China.
Appl Biochem Biotechnol. 2013 Oct;171(4):1072-83. doi: 10.1007/s12010-013-0361-5. Epub 2013 Aug 13.
To understand the effect of enhanced UV-B radiation and low-energy N(+) ion beam radiation on the response of photosynthesis, antioxidant enzymes, and lipid peroxidation in rice seedlings, Oryza sativa was exposed to three different doses of low-energy N(+) ion beam and enhanced UV-B alone and in combination. Enhanced UV-B caused a marked decline in some photosynthetic parameters (net photosynthetic rate, transpiration rate, and stomatal conductance) and photosynthetic pigments, whereas it induced an increase in hydrogen peroxide (H2O2) accumulation, the rate of superoxide radical production, and the content of malondialdehyde (MDA). Enhanced UV-B also induced an increase in the activity of antioxidant enzymes (superoxide dismutase [SOD], peroxidase (POD), and catalase [CAT]) and some nonenzymatic antioxidants such as proline. Under the combined treatment of enhanced UV-B and low-energy N(+) ion beam at the dose of 3.0 × 10(17) N(+) cm(-2), the activity of antioxidant compounds (SOD, POD, CAT, proline, and glutathione), photosynthetic pigments, and some photosynthetic parameters (net photosynthetic rate, transpiration rate, and stomatal conductance) increased significantly; however, the MDA content, H2O2 accumulation, and rate of superoxide radical production showed a remarkable decrease compared with the enhanced UV-B treatment alone. These results implied that the appropriate dose of low-energy N(+) ion beam treatment may alleviate the damage caused by the enhanced UV-B radiation on rice.
为了了解增强的 UV-B 辐射和低能 N(+)离子束辐射对水稻幼苗光合作用、抗氧化酶和脂质过氧化反应的影响，将水稻分别暴露在低能 N(+)离子束的三种不同剂量和增强的 UV-B 单独及联合辐射下。增强的 UV-B 导致一些光合作用参数（净光合速率、蒸腾速率和气孔导度）和光合色素显著下降，而诱导过氧化氢（H2O2）积累、超氧自由基产生速率和丙二醛（MDA）含量增加。增强的 UV-B 还诱导抗氧化酶（超氧化物歧化酶[SOD]、过氧化物酶[POD]和过氧化氢酶[CAT]）和一些非酶抗氧化剂（脯氨酸）活性增加。在 3.0×10(17) N(+) cm(-2)的低能 N(+)离子束剂量下，增强的 UV-B 和低能 N(+)离子束联合处理下，抗氧化化合物（SOD、POD、CAT、脯氨酸和谷胱甘肽）、光合色素和一些光合作用参数（净光合速率、蒸腾速率和气孔导度）的活性显著增加；然而，与单独增强的 UV-B 处理相比，MDA 含量、H2O2 积累和超氧自由基产生速率显著降低。这些结果表明，低能 N(+)离子束处理的适当剂量可能会减轻增强的 UV-B 辐射对水稻造成的损害。
