School of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan, 467044, Henan, China.
Appl Biochem Biotechnol. 2013 Oct;171(4):1061-71. doi: 10.1007/s12010-013-0417-6. Epub 2013 Aug 13.
The study aimed to evaluate the effects of deoxycholic acid (DCA) on human gastric carcinoma cell lines and to explore its mechanisms. In the present study, effects of DCA on SGC-7901 cell growth, cell cycle, and apoptosis were investigated by MTT assay, inverted microscopy, fluorescence microscopy, PI single- and FITC/PI double-staining flow cytometry, and western blotting. The study have revealed that DCA significantly inhibited the growth of SGC-7901 cells in a dose- and time-dependent manner and arrested cell cycle at G0/G1 phase. SGC-7901 cells showed typical apoptotic morphological changes after treated with DCA for 48 h. The intensity of typical apoptosis pattern- "ladders" formed by DNA in fragments of multiples of 200 base pairs was also observed. Apoptosis of SGC-7901 cells induced by DCA were associated with collapse of the mitochondrial membrane potential. DCA treatment could also increase the ratio of Bax to Bcl-2 in SGC-7901 cells. Meanwhile, the expression of p53, cyclinD1, and c-Myc were changed after DCA treatment. These results suggest that DCA induces apoptosis of gastric carcinoma cells through an intrinsic mitochondrial-dependent pathway, and the increase in the Bax/Bcl-2 ratio and collapse of the mitochondrial membrane potential may play important roles in DCA-induced apoptosis of gastric carcinoma cells.
本研究旨在评估脱氧胆酸（DCA）对人胃癌细胞系的影响，并探讨其作用机制。在本研究中，通过 MTT 法、倒置显微镜观察、荧光显微镜观察、PI 单染和 FITC/PI 双染流式细胞术以及 Western blot 分析，研究了 DCA 对 SGC-7901 细胞生长、细胞周期和凋亡的影响。结果表明，DCA 呈剂量和时间依赖性地显著抑制 SGC-7901 细胞的生长，并将细胞周期阻滞在 G0/G1 期。SGC-7901 细胞经 DCA 处理 48 h 后出现典型的凋亡形态学改变。还观察到由 DNA 片段形成的典型凋亡模式“梯状”，其片段为 200 个碱基对的倍数。DCA 诱导的 SGC-7901 细胞凋亡与线粒体膜电位崩溃有关。DCA 处理还可以增加 SGC-7901 细胞中 Bax 与 Bcl-2 的比值。同时，DCA 处理后 p53、cyclinD1 和 c-Myc 的表达发生变化。这些结果表明，DCA 通过内在的线粒体依赖性途径诱导胃癌细胞凋亡，Bax/Bcl-2 比值的增加和线粒体膜电位的崩溃可能在 DCA 诱导胃癌细胞凋亡中发挥重要作用。
