Institute of Theoretical and Experimental Biophysics (RAS), Pushchino, Moscow Region, 142290, Russia,
Mol Cell Biochem. 2013 Nov;383(1-2):261-9. doi: 10.1007/s11010-013-1774-8. Epub 2013 Aug 14.
The role of mitochondria in the inherited or ontogenetically acquired reactions of organism to stress is not studied enough. In the present work, we examined the functional state of the coupled respiratory chain, potassium and calcium transport and rate of hydrogen peroxide production on two rat lines: August and Wistar-which possess different resistance to emotional stress and hypoxia. It was established that the respiration rate and efficiency of oxidative phosphorylation were higher in August rats than in Wistar ones. In August rats, the rate of potassium transport and ATP-dependent mitochondrial swelling as well as the concentration of the ion in the mitochondrial matrix were almost twice as higher comparatively to those parameters in Wistar rats. The rate of H2O2 production was found to be decreased in the mitochondria of August rats. It was also demonstrated that the two rat lines differed by their resistance to the opening of the palmitate/Ca(2+)-induced pore and by their ability to retain calcium within mitochondria. The paper discusses the involvement of the mitochondrial ATP-dependent potassium channel in the adaptation of animals to adverse effects.
线粒体在机体对压力的遗传或个体发生获得性反应中的作用尚未得到充分研究。在本工作中，我们研究了具有不同抗情绪应激和缺氧能力的两个大鼠品系（August 和 Wistar）中线粒体耦联呼吸链、钾和钙转运以及过氧化氢产生率的功能状态。结果表明，August 大鼠的呼吸速率和氧化磷酸化效率高于 Wistar 大鼠。在 August 大鼠中，钾转运和 ATP 依赖性线粒体肿胀的速率以及线粒体基质中离子的浓度几乎是 Wistar 大鼠的两倍。发现 August 大鼠线粒体中 H2O2 的产生速率降低。还表明，这两个大鼠品系在对棕榈酸/Ca2+诱导的孔开放的抗性和在线粒体中保留钙的能力上存在差异。本文讨论了线粒体 ATP 依赖性钾通道在动物适应不利影响中的作用。
