Romero Adriana M, Mastromatteo-Alberga Patrizzia, Escalona Laura, Correnti María
Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela, Caracas, Venezuela.
Invest Clin. 2013 Jun;54(2):138-48.
Immune-inflammatory processes are trigged in chronic periodontitis (CP), where matrix metalloproteinases (MMPs) are released and involved in the degradation of the extracellular matrix components that can be detected in the gingival crevicular fluid (GCF). The purpose of the study was to determine the levels of MMP-3 and MMP-8 in GCF, before and after nonsurgical periodontal treatment (NSPT), to evaluate disease activity and therapy response. Eleven patients with PC and eleven healthy controls were selected. Clinical measurements to evaluate gingival index (GI), plaque index (PI), probing depth (PD) and clinical attachment loss (CAL) were made in all the teeth of each individual and in six sites per tooth. GCF samples were taken from one tooth per quadrant, with a pocket depth > or =4 mm and a clinical attachment loss > or =5 mm, and the levels of MMP-3 and MMP-8 measured using an ELISA test. Statistically significant differences in clinical parameters were observed (p < 0.05) between patients with CP and control groups before the periodontal treatment, with significant decrease in all indexes after the NSPT. The initial concentrations of MMP-3 and MMP-8 were significantly higher than those obtained after the NSPT and in the control group, without observing a correlation between the clinical parameters and the levels of MMPs. Increased levels of MMP-3 and MMP-8 in the GCF of patients with PC declined significantly after NSPT, and the difference between the levels in healthy individuals and patients, suggests the important participation of these MMPs in tissue destruction in PC disease..
慢性牙周炎（CP）中会引发免疫炎症过程，在此过程中会释放基质金属蛋白酶（MMPs），其参与细胞外基质成分的降解，这些成分可在龈沟液（GCF）中检测到。本研究的目的是测定非手术牙周治疗（NSPT）前后龈沟液中MMP - 3和MMP - 8的水平，以评估疾病活动度和治疗反应。选取了11例牙周炎患者和11例健康对照者。对每个个体的所有牙齿以及每颗牙齿的六个部位进行临床测量，以评估牙龈指数（GI）、菌斑指数（PI）、探诊深度（PD）和临床附着丧失（CAL）。从每个象限的一颗牙齿采集龈沟液样本，该牙齿的牙周袋深度≥4 mm且临床附着丧失≥5 mm，并使用酶联免疫吸附测定（ELISA）试验测量MMP - 3和MMP - 8的水平。牙周治疗前，CP患者与对照组之间在临床参数上观察到统计学显著差异（p < 0.05），NSPT后所有指标均显著下降。MMP - 3和MMP - 8的初始浓度显著高于NSPT后及对照组所测得的浓度，且未观察到临床参数与MMPs水平之间存在相关性。NSPT后，牙周炎患者龈沟液中MMP - 3和MMP - 8水平显著下降，健康个体与患者之间水平的差异表明这些MMPs在牙周炎疾病的组织破坏中起重要作用。
