Zhao Xiao-Ping, Guo Min, Zhang Bo-Li
Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1577-80.
Using fluorescein diacetate labeled HK-2 cells, a fast method for screening nephrotoxic components in traditional Chinese medicines (TCMs) was proposed in this study. The methodology validation showed that the linearity, stability and accuracy of the proposed method were suitable for screening nephrotoxic components in vitro . This method was further applied on screen 352 components from 32 Chinese Pharmacopoeia-indexed toxic TCMs. The results indicated that 31 components from 14 toxic TCMs, including Badou, had significant toxicity on HK-2 cells, which suggested these components may cause nephrotoxicity. The components from the other 18 toxic TCMs had no significant toxicity on HK-2 cells.
本研究利用荧光素二乙酸酯标记的HK-2细胞，提出了一种快速筛选中药肾毒性成分的方法。方法学验证表明，该方法的线性、稳定性和准确性适用于体外肾毒性成分的筛选。该方法进一步应用于对32种《中国药典》收载的有毒中药中的352种成分进行筛选。结果表明，包括巴豆在内的14种有毒中药中的31种成分对HK-2细胞具有显著毒性，提示这些成分可能导致肾毒性。其他18种有毒中药中的成分对HK-2细胞无显著毒性。
