Xarxa de Referencia de Recerca i Desenvolupament en Aquicultura de la Generalitat de Catalunya, Departament de Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona , Avinguda Diagonal 643, E-08028 Barcelona, Spain.
J Agric Food Chem. 2013 Sep 18;61(37):8924-33. doi: 10.1021/jf402617h. Epub 2013 Sep 9.
Ideal nutritional conditions are crucial to sustainable aquaculture due to economic and environmental issues. Here we apply stable isotope analysis as an indicator of fish growth and feeding balance, to define the optimum diet for efficient growing conditions. Juveniles of gilthead sea bream were fed with six isoenergetic diets differing in protein to lipid proportion (from 41/26 to 57/20). As protein intake increased, δ¹⁵N and Δδ¹⁵N of muscle and Δδ¹⁵N and Δδ¹³C of its protein fraction decreased, indicating lower protein turnover and higher protein deposition in muscle. This is reflected in the inverse relationship found between Δδ¹⁵N and growth rate, although no differences were observed in either parameter beyond the protein/lipid proportion 47/23. Principal component analysis (PCA) also signaled 47/23 diet as the pivotal point with the highest growing efficiency, with isotopic parameters having the highest discrimination load. Thus, muscle isotope composition, especially ¹⁵N, can be used to evaluate nutritional status in farmed fish.
由于经济和环境问题，理想的营养条件对可持续水产养殖至关重要。在这里，我们应用稳定同位素分析作为鱼类生长和摄食平衡的指标，来定义最佳的饮食以达到高效的生长条件。我们用六种等能量的饲料来饲养欧洲海鲈幼鱼，这些饲料的蛋白质与脂肪比例不同（从 41/26 到 57/20）。随着蛋白质摄入量的增加，肌肉中的 δ¹⁵N 和 Δδ¹⁵N 以及其蛋白质部分的 Δδ¹⁵N 和 Δδ¹³C 减少，表明肌肉中的蛋白质周转率降低，蛋白质沉积增加。这反映在发现 Δδ¹⁵N 与生长率之间的反比关系中，尽管在蛋白质/脂肪比例超过 47/23 后，这两个参数都没有差异。主成分分析（PCA）也表明 47/23 饮食是具有最高生长效率的关键点，同位素参数具有最高的区分负荷。因此，肌肉同位素组成，特别是 ¹⁵N，可以用于评估养殖鱼类的营养状况。
