Department of Chemistry, University of Illinois at Chicago , Chicago, Illinois 60607, United States.
ACS Appl Mater Interfaces. 2013 Oct 9;5(19):9269-75. doi: 10.1021/am4020633. Epub 2013 Aug 15.
Mass spectrometry (MS) imaging is increasingly being applied to probe the interfaces of biomaterials with invasive microbial biofilms, human tissue, or other biological materials. Laser desorption vacuum ultraviolet postionization with ∼75 fs, 800 nm laser pulses (fs-LDPI-MS) was used to collect MS images of a yeast-Escherichia coli co-culture biofilm. The method was also used to depth profile a three-dimensionally structured, multispecies biofilm. Finally, fs-LDPI-MS analyses of yeast biofilms grown under different conditions were compared with LDPI-MS using ultraviolet, nanosecond pulse length laser desorption as well as with fs laser desorption ionization without postionization. Preliminary implications for the use of fs-LDPI-MS for the analysis of biomaterials interfaces are discussed and contrasted with established methods in MS imaging.
质谱成像技术越来越多地被应用于探测生物材料与侵入性微生物生物膜、人体组织或其他生物材料的界面。利用 75 fs、800nm 激光脉冲（fs-LDPI-MS）的激光解吸真空紫外后电离技术，对酵母-大肠杆菌共培养生物膜进行了 MS 成像。该方法还用于对三维结构的多物种生物膜进行深度剖析。最后，比较了不同条件下生长的酵母生物膜的 fs-LDPI-MS 分析结果，与使用紫外线、纳秒脉冲长度激光解吸的 LDPI-MS 分析结果以及无后电离的 fs 激光解吸电离的分析结果。讨论了 fs-LDPI-MS 在生物材料界面分析中的初步应用，并与 MS 成像中的现有方法进行了对比。
