Department of Environmental Engineering , Korea Maritime University, Busan, Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2013;48(13):1732-9. doi: 10.1080/10934529.2013.815563.
A novel microbial consortium (BM-S-1) enriched from natural soils was successfully used to treat tannery wastewater from leather manufacturing industries in Korea on a pilot scale. The objective of this study was to determine whether augmentation with a novel microbial consortium BM-S-1could successfully treat the recalcitrant wastewater without chemical pre-treatment in a tannery wastewater treatment system. Chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) were monitored for water quality. The microbial population dynamics were analyzed using pyrosequencing, and denitrifying bacteria were quantified using real-time PCR (RT-PCR). The removal efficiencies for COD, TN and TP were greater than 91%, 79%, and 90%, respectively. The dominant phyla in the buffering tank (B), primary aeration (PA), secondary aeration (SA) and sludge digestion tank (SD) were Proteobacteria, Firmicutes, Bacteroidetes, Planctomycetes and Deinococcus-Thermus. Cluster analysis based on the UniFrac distance of the species in the different stages showed that the PA is similar to the SA, whereas the B is similar to the SD. qPCR of the nosZ genes showed the highest abundance of denitrifiers in B, which was increased 734-fold compared to the influent (I). It was hypothesized that anaerobic denitrifiers and the diverse microbial community may play important roles in the biological treatment of tannery wastewater. This technology may also contribute to the full-scale treatment of industrial wastewater containing food processing wastewater and marine sediment with high organic content.
从天然土壤中富集的新型微生物联合体（BM-S-1）成功地用于在中试规模上处理来自韩国皮革制造行业的制革废水。本研究的目的是确定在制革废水处理系统中，添加新型微生物联合体 BM-S-1 是否可以在无需化学预处理的情况下成功处理难处理的废水。监测水质的化学需氧量（COD）、总氮（TN）和总磷（TP）。使用焦磷酸测序分析微生物种群动态，并使用实时 PCR（RT-PCR）定量测定反硝化细菌。COD、TN 和 TP 的去除效率分别大于 91%、79%和 90%。缓冲罐（B）、初级曝气（PA）、二级曝气（SA）和污泥消化罐（SD）中的优势菌群分别为变形菌门、厚壁菌门、拟杆菌门、浮霉菌门和古菌门。基于不同阶段物种的 UniFrac 距离的聚类分析表明，PA 与 SA 相似，而 B 与 SD 相似。nosZ 基因的 qPCR 显示 B 中反硝化菌的丰度最高，与进水（I）相比增加了 734 倍。据推测，厌氧反硝化菌和多样化的微生物群落可能在制革废水的生物处理中发挥重要作用。该技术还可能有助于处理含有高有机含量的食品加工废水和海洋沉积物的工业废水的全规模处理。
