人类卵泡卵母细胞对孤雌激活的抗性：体外培养卵母细胞中的DNA分布与含量
Resistance of human follicular oocytes to parthenogenetic activation: DNA distribution and content in oocytes maintained in vitro.
作者信息
Abramczuk J W, Lopata A
机构信息
Royal Women's Hospital, Carlton, Australia.
出版信息
Hum Reprod. 1990 Jul;5(5):578-81. doi: 10.1093/oxfordjournals.humrep.a137148.
Abstract
Sixty-nine human oocytes were subjected to various parthenogenetic stimuli but no activation was observed. Analysis of Feulgen-stained DNA (F-DNA) distribution and content showed anomalies of chromosomal material in 36% of oocytes at 24-75 h after retrieval. A significant loss of F-DNA was noticed in apparently normal metaphase II oocytes remaining in culture for 2-3 days.
摘要
69个人类卵母细胞接受了各种孤雌生殖刺激，但未观察到激活现象。对Feulgen染色的DNA（F-DNA）分布和含量的分析表明，在取卵后24至75小时，36%的卵母细胞存在染色体物质异常。在培养2至3天的明显正常的中期II卵母细胞中，发现F-DNA有显著损失。
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