Gook D A, Osborn S M, Johnston W I
Reproductive Biology Unit, Royal Women's Hospital, Carlton, Victoria, Australia.
Hum Reprod. 1995 Mar;10(3):654-8. doi: 10.1093/oxfordjournals.humrep.a136005.
Fresh and aged human oocytes were cryopreserved using 1,2-propanediol (PROH). After thawing, the oocytes were cultured for 20 h and examined for parthenogenetic activation using light microscopy and an ultraviolet DNA stain. Control fresh or aged oocytes and oocytes exposed to PROH without cryoperservation were also examined for activation. No control oocytes were observed to activate spontaneously (n = 43) and parthenogenetic activation was not induced by exposure to PROH alone (n = 26). In both fresh and aged cryopreserved oocytes, 27 and 29% of the oocytes respectively were activated, and these proportions were significantly elevated compared with the controls (P < 0.01). Although a similar rate of activation was observed for the cryopreserved fresh and aged oocytes, the form of parthenogenetic activation varied between these two types of oocyte. A single pronucleus was observed in 18% of the fresh and 5% of the aged cryopreserved oocytes. In contrast, the presence of two or more pronuclei was observed in 0% of the fresh and 19% of the aged cryopreserved oocytes.
使用1,2 - 丙二醇（PROH）对新鲜和老化的人类卵母细胞进行冷冻保存。解冻后，将卵母细胞培养20小时，并使用光学显微镜和紫外线DNA染色检查孤雌激活情况。还对未进行冷冻保存的对照新鲜或老化卵母细胞以及暴露于PROH的卵母细胞进行了激活检查。未观察到对照卵母细胞自发激活（n = 43），单独暴露于PROH也未诱导孤雌激活（n = 26）。在新鲜和老化的冷冻保存卵母细胞中，分别有27%和29%的卵母细胞被激活，与对照组相比，这些比例显著升高（P < 0.01）。尽管冷冻保存的新鲜和老化卵母细胞的激活率相似，但这两种类型的卵母细胞之间孤雌激活的形式有所不同。在18%的新鲜和5%的老化冷冻保存卵母细胞中观察到单个原核。相比之下，在0%的新鲜和19%的老化冷冻保存卵母细胞中观察到两个或更多原核。
