水杨酸增加盐胁迫小麦幼苗中谷胱甘肽和抗坏血酸的含量，并暂时调节相关基因的表达。
Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt-stressed wheat seedlings.
机构信息
The National Engineering Research Centre for Wheat, The Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China.
出版信息
Gene. 2013 Oct 25;529(2):321-5. doi: 10.1016/j.gene.2013.07.093. Epub 2013 Aug 13.
Exogenous salicylic acid (SA) significantly improved abiotic tolerance in higher plants, and ascorbate (ASA) and glutathione (GSH) play important roles in abiotic tolerance. In this study, SA (0.5mM) markedly increased the contents of ASA and GSH in SA-treated plants during salt stress (250mM NaCl). The transcript levels of the genes encoding ASA and GSH cycle enzymes were measured using quantitative real-time PCR. The results indicated that, during salt stress, exogenous SA significantly enhanced the transcripts of glutathione peroxidase (GPX1), phospholipid hydroperoxide glutathione peroxidase (GPX2) and dehydroascorbate reductase (DHAR) genes at 12h, glutathione reductase (GR) at 24h, 48h and 72h, glutathione-S-transferase 1 (GST1), 2 (GST2), monodehydroascorbate reductase (MDHAR) and glutathione synthetase (GS) at the 48h and 72h after salt stress, respectively. The results implied that SA temporally regulated the transcript levels of the genes encoding ASA-GSH cycle enzymes, resulting in the increased contents of GSH and ASA and enhanced salt tolerance.
外源水杨酸（SA）显著提高了高等植物的非生物胁迫耐受性，而抗坏血酸（ASA）和谷胱甘肽（GSH）在非生物胁迫耐受性中发挥着重要作用。在这项研究中，在盐胁迫（250mM NaCl）下，SA（0.5mM）显著增加了 SA 处理植物中 ASA 和 GSH 的含量。使用实时定量 PCR 测量了编码 ASA 和 GSH 循环酶的基因的转录水平。结果表明，在盐胁迫下，外源 SA 分别在 12h、24h、48h 和 72h 显著增强了谷胱甘肽过氧化物酶（GPX1）、磷脂氢过氧化物谷胱甘肽过氧化物酶（GPX2）和脱氢抗坏血酸还原酶（DHAR）基因的转录水平，在 48h 和 72h 分别增强了谷胱甘肽还原酶（GR）、谷胱甘肽-S-转移酶 1（GST1）、2（GST2）、单脱氢抗坏血酸还原酶（MDHAR）和谷胱甘肽合成酶（GS）的转录水平。结果表明，SA 暂时调节了编码 ASA-GSH 循环酶的基因的转录水平，导致 GSH 和 ASA 含量增加，增强了耐盐性。
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