Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46071 Valencia, Spain.
Biosens Bioelectron. 2014 Jan 15;51:109-14. doi: 10.1016/j.bios.2013.07.045. Epub 2013 Jul 29.
The potential and the capabilities of Blu-ray technology (discs and drives) for massive screening applications are presented. High density microarrays are fabricated onto a Blu-ray disc and applied for the determination of microcystin residues and pathogenic microorganisms. Specific probes were physisorbed onto the BD surface and the biorecognition event was displayed using labeled secondary antibody solution and subsequent signal amplification. The attenuation of the reflected light caused by the reaction product is detected by the Blu-ray drive and inversely correlated with analyte concentration. BDs preserve the optical properties according to Blu-ray specifications, ensuring maximum accuracy and sensitivity of the drive during disc scanning. Detection limits of 0.4 μg/L for microcystin LR and 10(0) and 10(1) cfu/mL for Salmonella typhimurium and Cronobacter sakazakii respectively, were achieved, improving considerably the DVD performances and reaching similar sensitivity as real-time quantitative PCR. Blu-ray technology adapted to the analysis of high density arrays highlights the enormous capabilities (high sensitivity, speed-scanning, optical resolution, portability) for point-of-care settings, diagnostics, and high-throughput screening applications.
本文介绍了蓝光技术（光盘和驱动器）在大规模筛选应用中的潜力和功能。将高密度微阵列制作到蓝光光盘上，并应用于微囊藻毒素残留和病原微生物的测定。将特异性探针物理吸附到 BD 表面上，并使用标记的二级抗体溶液和随后的信号放大来显示生物识别事件。反应产物引起的反射光的衰减由蓝光驱动器检测，并与分析物浓度成反比。BD 按照蓝光规格保留光学特性，确保在光盘扫描过程中驱动器具有最大的准确性和灵敏度。分别实现了对微囊藻毒素 LR 的检测限为 0.4μg/L，对鼠伤寒沙门氏菌和阪崎克罗诺杆菌的检测限为 10(0)和 10(1)cfu/mL，大大提高了 DVD 的性能，达到了与实时定量 PCR 相似的灵敏度。适用于高密度阵列分析的蓝光技术突出了其在即时检测、诊断和高通量筛选应用中的巨大潜力（高灵敏度、快速扫描、光学分辨率、便携性）。
