Jiang Jianye, Liu Sheng, Luo Jingjing, Li Xiang, Tang Shuangyang, Yu Minjun, Cai Hengling, Tian Tian, Zhang Yan
Institute of Pathobiology, University of South China, Hengyang, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):785-8.
To study the expressions of NLRP3 inflammasome and its downstream molecules in the gastric tissues and sera of Helicobacter pylori (H.pylori)-infected C57BL/6 mice, and to preliminary explore the role of NLRP3 inflammasome signalling pathway in the pathogenesis of H.pylori infection.
C57BL/6 mice were randomly divided into H.pylori infection group and PBS control group, and mice were sacrificed at different time points after H.pylori infection. The mRNA expressions of NLRP3 inflammasome and downstream signalling pathway related molecules in mouse gastric tissues were detected by RT-PCR, and the protein level of caspase-1 was analyzed by Western blotting. The contents of IL-1β, IL-18 and IL-33 in the sera were measured with ELISA.
Chronic inflammatory response was observed in the gastric mucosal tissues of H.pylori-infected mice and it gradually aggravated. Compared with the control mice, the mRNA expressions of NLRP3 inflammasome signalling pathway related molecules and the protein level of caspase-1 increased markedly in the gastric tissues of H.pylori-infected mice. Moreover, the contents of IL-1β, IL-18 and IL-33 in the sera of H.pylori-infected mice were also significantly elevated.
NLRP3 inflammasome signalling pathway could be activated by H.pylori infection.
研究幽门螺杆菌（H.pylori）感染的C57BL/6小鼠胃组织及血清中NLRP3炎性小体及其下游分子的表达情况，并初步探讨NLRP3炎性小体信号通路在H.pylori感染发病机制中的作用。
将C57BL/6小鼠随机分为H.pylori感染组和PBS对照组，在H.pylori感染后的不同时间点处死小鼠。采用RT-PCR检测小鼠胃组织中NLRP3炎性小体及下游信号通路相关分子的mRNA表达，并用Western blotting分析caspase-1的蛋白水平。采用ELISA检测血清中IL-1β、IL-18和IL-33的含量。
观察到H.pylori感染小鼠的胃黏膜组织出现慢性炎症反应，且炎症反应逐渐加重。与对照小鼠相比，H.pylori感染小鼠胃组织中NLRP3炎性小体信号通路相关分子的mRNA表达及caspase-1的蛋白水平显著升高。此外，H.pylori感染小鼠血清中IL-1β、IL-18和IL-33的含量也明显升高。
H.pylori感染可激活NLRP3炎性小体信号通路。
