Wang Lijuan, Shi Shengjia, Wang Le, Xie Yanju, Bai E, Zhou Xia, Li Meng, Jin Guihua, Zhu Qing
Department of Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):789-93.
To investigate the role of long non-coding RNA prostate cancer non-coding RNA1 (PRNCR1) in the castration resistant prostate cancer cells.
We compared the PRNCR1 mRNA expression of androgen-dependent prostate cancer cells LNCaP and androgen-independent prostate cancer cells C4-2 by real-time quantitative PCR (qRT-PCR). According to PRNCR1 gene sequence, siRNA fragments (PRNCR1-siRNA) were designed and synthesized to transfect C4-2 cells, and 48 h later, the expression of PRNCR1 mRNA was detected again by qRT-PCR to confirm the silence of PRNCR1. Thereafter, we determined the expression level of androgen receptor (AR) using Western blotting, and observed the change in the proliferation, apoptosis and invasion ability of C4-2 cells by means of MTT, flow cytometry and Transwell cell invasion assay, respectively.
Compared with LNCaP cells, the expression level of PRNCR1 mRNA in C4-2 cells increased significantly. After transfected with PRNCR1-siRNA to silence the PRNCR1 mRNA expression, the C4-2 cells showed the inhibited expression of AR protein, the depressed proliferation and invasion abilities and the increased apoptosis rate.
PRNCR1 may play an important role in the progression of castration resistant prostate cancer through mediating the expression of AR.
探讨长链非编码RNA前列腺癌非编码RNA1（PRNCR1）在去势抵抗性前列腺癌细胞中的作用。
我们通过实时定量PCR（qRT-PCR）比较雄激素依赖性前列腺癌细胞LNCaP和雄激素非依赖性前列腺癌细胞C4-2中PRNCR1 mRNA的表达。根据PRNCR1基因序列，设计并合成小干扰RNA片段（PRNCR1-siRNA）转染C4-2细胞，48小时后，再次通过qRT-PCR检测PRNCR1 mRNA的表达以确认PRNCR1的沉默。此后，我们使用蛋白质免疫印迹法测定雄激素受体（AR）的表达水平，并分别通过MTT法、流式细胞术和Transwell细胞侵袭试验观察C4-2细胞增殖、凋亡和侵袭能力的变化。
与LNCaP细胞相比，C4-2细胞中PRNCR1 mRNA的表达水平显著升高。用PRNCR1-siRNA转染使PRNCR1 mRNA表达沉默后，C4-2细胞显示出AR蛋白表达受抑制、增殖和侵袭能力降低以及凋亡率增加。
PRNCR1可能通过介导AR的表达在去势抵抗性前列腺癌进展中起重要作用。
