Liu Pingping, Xuan Xiaoyan, Zhu Jingsong, Guan Shasha, Du Ying, Li Qianru
Department of Immunology and Microbiology, Basic Medical College, Zhengzhou University, Zhengzhou, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):798-801.
To investigate the therapeutic effect of allogenic bone marrow stem cells (BMSCs) transplantation on experimental autoimmune encephalomyelitis (EAE) and the underlying immunoregulatory mechanism.
EAE models were established by myelin oligodendrocyte glycoprotein (MOG) peptide immunization in C57BL/6J mice; BMSCs were purified and cultured from bone marrow of BALB/c mice, then transplanted to the EAE models. The scores of neurological function defect were assessed before and after BMSCs transplantation. The frequencies of CD4(+);CD25(+);Foxp3(+); T cells (Tregs) in mice lymph organs were measured by flow cytometry and the expressions of IL-2, IL-4, IL-17 and IL-23 mRNA in mouse spleen samples were detected by real-time quantitative RT-PCR after BMSCs transplantation.
Transplantation of allogenic BMSCs improved the clinical score of the EAE mice. Compared with EAE control group, the frequencies of Tregs in spleen, lymph node and thymus of EAE mice transplanted with BMSCs increased significantly, and the levels of IL-2 and IL-17 mRNA significantly decreased, while IL-4 and IL-23 mRNA increased.
Transplantation of allogenic BMSCs can prevent the development of EAE by regulating the frequency of Tregs and the levels of the cytokines secreted by CD4(+);T cells.
探讨同种异体骨髓干细胞（BMSCs）移植对实验性自身免疫性脑脊髓炎（EAE）的治疗作用及其潜在的免疫调节机制。
通过用髓鞘少突胶质细胞糖蛋白（MOG）肽免疫C57BL/6J小鼠建立EAE模型；从BALB/c小鼠骨髓中纯化并培养BMSCs，然后将其移植到EAE模型中。在BMSCs移植前后评估神经功能缺损评分。通过流式细胞术检测小鼠淋巴器官中CD4(+)、CD25(+)、Foxp3(+) T细胞（调节性T细胞，Tregs）的频率，并在BMSCs移植后通过实时定量RT-PCR检测小鼠脾脏样本中IL-2、IL-4、IL-17和IL-23 mRNA的表达。
同种异体BMSCs移植改善了EAE小鼠的临床评分。与EAE对照组相比，移植BMSCs的EAE小鼠脾脏、淋巴结和胸腺中Tregs的频率显著增加，IL-2和IL-17 mRNA水平显著降低，而IL-4和IL-23 mRNA增加。
同种异体BMSCs移植可通过调节Tregs频率和CD4(+) T细胞分泌的细胞因子水平来预防EAE的发展。
