[CXCR4(+)骨髓间充质干细胞的迁移及氧化型低密度脂蛋白诱导的内皮细胞损伤]

[The migration of CXCR4(+); BMSCs and the ox-LDL-induced injury in endothelial cells].

作者信息

Yu Gang, Xia Hongli, Li Mincai

机构信息

School of Basic Medicine, Xianning Central Hospital, Hubei University of Science and Technology, Xianning, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):805-8.

PMID:23948404

Abstract

OBJECTIVE

To study whether the stromal cell-derived factor 1 (SDF-1) secreted by endothelial cells affects the CXCR4(+); stem cell migration.

METHODS

CXCR4(+); bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from mouse bone marrow. Human umbilical vein endothelial cells (HUVECs) were stimulated with oxidized low-density lipoprotein (ox-LDL), and the cell proliferation was detected by MTT assay, the expressions of SDF-1α mRNA and protein were detected by RT-PCR and ELISA, respectively. The migration of CXCR4(+); BMSCs was analyzed by Transwell(R); chamber assay.

RESULTS

The stimulation of ox-LDL affected the proliferation and increased significantly the expression levels of SDF-1α mRNA and protein in HUVECs, and the medium supernatant promoted the migratory response of CXCR4(+); BMSCs. When the neutralizing CXCR4 antibody eliminated the secreted SDF-1α, the migratory activity markedly decreased.

CONCLUSION

CXCR4(+); BMSCs might migrate to endothelial cells by SDF-1α/CXCR4 axis in the atherosclerosis process.

摘要

目的

研究内皮细胞分泌的基质细胞衍生因子1（SDF-1）是否影响CXCR4（+）干细胞迁移。

方法

从小鼠骨髓中分离出CXCR4（+）骨髓间充质干细胞（BMSCs）。用氧化型低密度脂蛋白（ox-LDL）刺激人脐静脉内皮细胞（HUVECs），采用MTT法检测细胞增殖，分别用RT-PCR和ELISA检测SDF-1α mRNA和蛋白的表达。通过Transwell小室分析法分析CXCR4（+）BMSCs的迁移。