Yu Gang, Xia Hongli, Li Mincai
School of Basic Medicine, Xianning Central Hospital, Hubei University of Science and Technology, Xianning, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):805-8.
To study whether the stromal cell-derived factor 1 (SDF-1) secreted by endothelial cells affects the CXCR4(+); stem cell migration.
CXCR4(+); bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from mouse bone marrow. Human umbilical vein endothelial cells (HUVECs) were stimulated with oxidized low-density lipoprotein (ox-LDL), and the cell proliferation was detected by MTT assay, the expressions of SDF-1α mRNA and protein were detected by RT-PCR and ELISA, respectively. The migration of CXCR4(+); BMSCs was analyzed by Transwell(R); chamber assay.
The stimulation of ox-LDL affected the proliferation and increased significantly the expression levels of SDF-1α mRNA and protein in HUVECs, and the medium supernatant promoted the migratory response of CXCR4(+); BMSCs. When the neutralizing CXCR4 antibody eliminated the secreted SDF-1α, the migratory activity markedly decreased.
CXCR4(+); BMSCs might migrate to endothelial cells by SDF-1α/CXCR4 axis in the atherosclerosis process.
研究内皮细胞分泌的基质细胞衍生因子1（SDF-1）是否影响CXCR4（+）干细胞迁移。
从小鼠骨髓中分离出CXCR4（+）骨髓间充质干细胞（BMSCs）。用氧化型低密度脂蛋白（ox-LDL）刺激人脐静脉内皮细胞（HUVECs），采用MTT法检测细胞增殖，分别用RT-PCR和ELISA检测SDF-1α mRNA和蛋白的表达。通过Transwell小室分析法分析CXCR4（+）BMSCs的迁移。
ox-LDL刺激影响了HUVECs的增殖，并显著提高了SDF-1α mRNA和蛋白的表达水平，且培养基上清液促进了CXCR4（+）BMSCs的迁移反应。当用中和性CXCR4抗体消除分泌的SDF-1α时，迁移活性明显降低。
在动脉粥样硬化过程中，CXCR4（+）BMSCs可能通过SDF-1α/CXCR4轴迁移至内皮细胞。
