Department of Nursing I, School of Nursing, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain; Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain; BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain.
Transl Res. 2013 Nov;162(5):297-308. doi: 10.1016/j.trsl.2013.07.010. Epub 2013 Aug 13.
Aspartyl aminopeptidase (ASP; EC 3.4.11.21) is a widely distributed and abundant cytosolic enzyme that regulates bioactive peptides such as angiotensin II. It has been demonstrated that the expression and activity of this enzyme is modified in tissue and serum of patients with several types of cancer. However, the involvement of ASP in the neoplastic development and survival of patients with colorectal cancer (CRC) has not been analyzed to date. The activity and messenger RNA expression of ASP in tumor tissue (n = 71) and plasma (n = 40) of patients with CRC was analyzed prospectively using fluorometric and quantitative real-time polymerase chain reaction methods. Data obtained from tumor tissue were compared with those from the surrounding normal mucosa. Classic pathologic parameters (grade, stage, nodal invasion, distant metastases and perineural, lymphatic, and vascular invasion) were stratified following ASP data and analyzed for 5-year survival. ASP was upregulated in CRC tissues, and greater activity correlated significantly with the absence of lymph node metastases and with better overall survival. Inversely, greater plasmatic ASP activity was associated with worse overall and disease-free survival. Data suggest that ASP is involved in colorectal neoplasia and point to this enzyme as a potential useful diagnostic tool in clinical practice.
天冬氨酰氨基肽酶（ASP；EC 3.4.11.21）是一种广泛分布且丰富的细胞质酶，可调节血管紧张素 II 等生物活性肽。已经证明，这种酶的表达和活性在患有多种类型癌症的患者的组织和血清中发生了改变。然而，迄今为止，ASP 参与结直肠癌（CRC）患者的肿瘤发生和存活尚未得到分析。使用荧光法和定量实时聚合酶链反应方法，前瞻性地分析了 CRC 患者肿瘤组织（n=71）和血浆（n=40）中 ASP 的活性和信使 RNA 表达。从肿瘤组织获得的数据与周围正常黏膜的数据进行了比较。根据 ASP 数据对经典病理参数（分级、分期、淋巴结浸润、远处转移以及神经周围、淋巴和血管浸润）进行分层，并分析 5 年生存率。CRC 组织中 ASP 上调，其活性增加与无淋巴结转移和总体生存率提高显著相关。相反，更高的血浆 ASP 活性与总体生存率和无病生存率降低相关。数据表明，ASP 参与了结直肠肿瘤的发生，并指出该酶是临床实践中一种有潜在应用价值的诊断工具。
