Second Department of Surgery, Wakayama Medical University, School of Medicine, Wakayama, Japan.
J Surg Res. 2013 Nov;185(1):136-42. doi: 10.1016/j.jss.2013.05.041. Epub 2013 Jun 2.
The identification of molecular markers that are useful for predicting lymph node metastasis is urgently needed to determine treatment strategies for T1 colorectal cancer (CRC). We previously showed that 10 candidate genes are correlated with de-differentiation at the invasion front of CRC using a gene expression analysis. These 10 genes are potential markers that may predict lymph node metastasis by CRC.
Samples were obtained from 161 patients with CRC. Quantitative real-time reverse transcription-polymerase chain reaction assays were performed using 66 T3 samples in order to extract genes correlated with lymph node metastasis. Immunohistochemical studies of the extracted genes were performed on 66 T3 and 95 T1 samples. A univariate analysis followed by a multivariate logistic regression model was used to examine independent risk factors for lymph node metastasis.
The CITED1 messenger RNA expression was found to be an independent risk factor for lymph node metastasis in T3 CRC patients (P = 0.040). A high CITED1 protein expression, as detected with immunohistochemistry, was also an independent risk factor in T3 CRC patients (P = 0.035). In T1 colorectal cancer patients, a high CITED1 protein expression was found to be an independent risk factor for lymph node metastasis (P = 0.010). The positive predictive and negative predictive values in the T1 colorectal cancer patients were 27.5% and 95.5%, respectively.
The CITED1 expression is correlated with lymph node metastasis in patients with CRC. In T1 colorectal cancer patients, CITED1 has the potential ability to predict the presence of lymph node metastasis.
为了确定 T1 结直肠癌（CRC）的治疗策略，迫切需要鉴定出有助于预测淋巴结转移的分子标志物。我们之前曾使用基因表达分析显示，在 CRC 的侵袭前沿存在与去分化相关的 10 个候选基因。这 10 个基因可能是预测 CRC 淋巴结转移的潜在标志物。
从 161 例 CRC 患者中获取样本。对 66 例 T3 样本进行定量实时逆转录-聚合酶链反应（qRT-PCR）检测，以提取与淋巴结转移相关的基因。对 66 例 T3 和 95 例 T1 样本中提取的基因进行免疫组织化学研究。采用单因素分析和多变量逻辑回归模型来检查淋巴结转移的独立危险因素。
发现 CITED1 信使 RNA 表达是 T3CRC 患者淋巴结转移的独立危险因素（P = 0.040）。免疫组化检测到的 CITED1 蛋白高表达也是 T3CRC 患者的独立危险因素（P = 0.035）。在 T1 结直肠癌患者中，CITED1 蛋白高表达是淋巴结转移的独立危险因素（P = 0.010）。在 T1 结直肠癌患者中，阳性预测值和阴性预测值分别为 27.5%和 95.5%。
CITED1 表达与 CRC 患者的淋巴结转移相关。在 T1 结直肠癌患者中，CITED1 具有预测淋巴结转移的潜在能力。
