G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia.
Photosynth Res. 2013 Sep;116(1):55-78. doi: 10.1007/s11120-013-9896-0. Epub 2013 Aug 15.
The values of gross metabolic flows in cells are essentially interconnected due to conservation laws of chemical elements and interrelations of biochemical coupling. Therefore, the overall stoichiometry of cellular metabolism, such as the biomass quantum yield, the ratio between linear and circular flows via the electron transport chain, etc., can be calculated using balances of metabolic flows in the network branching points and coupling ratios related to ATP formation and expenditures. This work has studied the energetic stoichiometry of photosynthetic cells by considering the transfer of reductivity in the course of biochemical reactions. This approach yielded rigorous mathematical expressions for biomass quantum yield and other integral bioenergetic indices of cellular growth as functions of ATP balance parameters. The effect of cellular substance turnover has been taken into account. The obtained theoretical estimation of biomass quantum yield is rather close to experimental data which confirms the predictive capacity of this approach.
由于化学元素的守恒定律和生化耦联的相互关系，细胞中总代谢通量的数值本质上是相互关联的。因此，细胞代谢的整体化学计量学，如生物量量子产率、通过电子传递链的线性和循环流之间的比例等，可以通过网络分支点的代谢通量平衡和与 ATP 形成和消耗相关的耦合比来计算。这项工作通过考虑生化反应过程中还原性的转移来研究光合细胞的能量化学计量学。这种方法为生物量量子产率和细胞生长的其他整体生物能学指标提供了严格的数学表达式，这些指标是作为 ATP 平衡参数的函数。已经考虑了细胞物质周转率的影响。所得生物量量子产率的理论估计值与实验数据相当接近，这证实了该方法的预测能力。
