Monje Lucas D, Quiroga Martín, Manzoli Darío, Couri Márcia S, Silvestri Leonardo, Venzal José M, Cuervo Pablo, Beldomenico Pablo M
Laboratorio de Ecología de Enfermedades, Instituto de Ciencias Veterinarias del Litoral, Universidad Nacional del Litoral-Consejo Nacional de Investigaciones Científicas y Tecnológicas, Santa Fe, Argentina.
Syst Parasitol. 2013 Sep;86(1):43-51. doi: 10.1007/s11230-013-9428-5. Epub 2013 Aug 15.
Philornis Meinert, 1890 (Diptera: Muscidae) is a genus of Neotropical dipterans that parasitise birds. The currently used external morphological characters to distinguish between species within this genus present some limitations. We used the second internal transcribed spacer region (ITS2) of the rRNA gene as a molecular marker to differentiate adult specimens of Philornis identified morphologically as Philornis torquans and Philornis seguyi from different localities. Specimens identified as P. seguyi from Magdalena (Buenos Aires Province) showed an ITS2 sequence different from that for P. torquans, whereas all other specimens of P. seguyi had sequences identical to those for P. torquans. These findings do not necessarily confirm that specimens from Magdalena indeed belong to P. seguyi, nor that P. seguyi is a valid species. Instead, they alert us about the potential for species misidentification when using morphological characters alone. The use of molecular approaches to aid the identification of Philornis spp. will shed light on the systematics of this group. P. torquans is reported for the first time in Mendoza Province and Uruguay.
菲尔诺氏蝇（Philornis Meinert，1890年）（双翅目：蝇科）是一类寄生于鸟类的新热带区双翅目昆虫。目前用于区分该属内物种的外部形态特征存在一些局限性。我们使用核糖体RNA基因的第二内部转录间隔区（ITS2）作为分子标记，来区分形态学上鉴定为扭形菲尔诺氏蝇（Philornis torquans）和塞氏菲尔诺氏蝇（Philornis seguyi）的来自不同地区的成年标本。从马格达莱纳（布宜诺斯艾利斯省）鉴定为塞氏菲尔诺氏蝇的标本显示出与扭形菲尔诺氏蝇不同的ITS2序列，而塞氏菲尔诺氏蝇的所有其他标本具有与扭形菲尔诺氏蝇相同的序列。这些发现不一定证实来自马格达莱纳的标本确实属于塞氏菲尔诺氏蝇，也不能证实塞氏菲尔诺氏蝇是一个有效物种。相反，它们提醒我们仅使用形态特征时存在物种误认的可能性。使用分子方法辅助鉴定菲尔诺氏蝇属物种将为该类群的系统学研究提供线索。扭形菲尔诺氏蝇首次在门多萨省和乌拉圭被报道。
