Mao Jianjun, Zeng Fanrong
The Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Transgenic Res. 2014 Feb;23(1):145-52. doi: 10.1007/s11248-013-9739-y. Epub 2013 Aug 15.
Plant-mediated RNAi has been developed as a powerful weapon in the fight against agricultural insect pests. The gap gene hunchback (hb) is of crucial importance in insect axial patterning and knockdown of hb is deforming and lethal to the next generation. The peach potato aphid, Myzus persicae (Sulzer), has many host plants and can be found throughout the world. To investigate the effect of plant-mediated RNAi on control of this insect, the hb gene in M. persicae was cloned, plant RNAi vector was constructed, and transgenic tobacco expressing Mphb dsRNA was developed. Transgenic tobacco had a different integration pattern of the transgene. Bioassays were performed by applying neonate aphids to homozygous transgenic plants in the T2 generation. Results revealed that continuous feeding of transgenic diet reduced Mphb mRNA level in the fed aphids and inhibited insect reproduction, indicating successful knockdown of the target gene in M. persicae by plant-mediated RNAi.
植物介导的RNA干扰已发展成为对抗农业害虫的有力武器。间隙基因驼背（hb）在昆虫轴向模式形成中至关重要，敲低hb会导致畸形并对下一代具有致死性。桃蚜，烟蚜（Myzus persicae (Sulzer)），有许多寄主植物，遍布世界各地。为了研究植物介导的RNA干扰对这种昆虫的防治效果，克隆了烟蚜中的hb基因，构建了植物RNA干扰载体，并培育出表达Mphb dsRNA的转基因烟草。转基因烟草具有不同的转基因整合模式。通过将新生蚜虫应用于T2代纯合转基因植物进行生物测定。结果表明，持续取食转基因食物会降低取食蚜虫中Mphb mRNA水平并抑制昆虫繁殖，表明通过植物介导的RNA干扰成功敲低了烟蚜中的靶基因。
