Department of Internal Medicine, GangNeung Asan Medical Center, University of Ulsan College of Medicine, Gangneung, Korea.
Gastric Cancer. 2014;17(3):402-11. doi: 10.1007/s10120-013-0288-0. Epub 2013 Aug 17.
The aim of this study was to use immunohistochemistry (IHC) and silver in situ hybridization (SISH) to evaluate alterations in EGFR and HER2 in gastric cancer in order to determine the relationship with prognosis in gastric cancer patients following curative resection.
In this study, we analyzed EGFR and HER-2 status by IHC and SISH in 254 stage I-III gastric cancer patients who underwent curative surgery.
Thirteen cases (2.48 %) showed EGFR alteration by IHC or SISH. EGFR alteration was associated with older age (P = 0.021), intestinal type (P = 0.040) and higher stage disease (P < 0.001). The patients with operable state gastric cancer who had EGFR alteration had an unfavorable prognosis, and multivariate analysis confirmed that EGFR alteration was an independent unfavorable prognostic factor. Twenty-seven cases (10.6 %) showed HER-2 alteration by IHC or SISH. HER-2 alteration was associated with older age (P = 0.006), well or moderately differentiated histology (P < 0.001) and intestinal type (P = 0.002).
HER-2 alteration is not an independent prognostic factor for curatively resectable gastric cancer. We observed EGFR alteration in a subset of cases with operable state gastric cancer and determined that it was associated with an unfavorable prognosis.
本研究旨在通过免疫组织化学（IHC）和原位杂交银染（SISH）评估胃癌中 EGFR 和 HER2 的改变，以确定其与可切除胃癌患者根治性切除术后预后的关系。
本研究分析了 254 例接受根治性手术的 I 期至 III 期胃癌患者的 EGFR 和 HER-2 状态。
13 例（2.48%）IHC 或 SISH 显示 EGFR 改变。EGFR 改变与年龄较大（P=0.021）、肠型（P=0.040）和较高的疾病分期（P<0.001）有关。可切除状态的胃癌患者 EGFR 改变与预后不良相关，多因素分析证实 EGFR 改变是独立的预后不良因素。27 例（10.6%）IHC 或 SISH 显示 HER-2 改变。HER-2 改变与年龄较大（P=0.006）、高或中分化组织学（P<0.001）和肠型（P=0.002）有关。
HER-2 改变不是可切除胃癌的独立预后因素。我们观察到一部分可切除胃癌病例存在 EGFR 改变，并确定其与预后不良有关。
