L. N. Ivanova: Institute of Cytology and Genetics Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
Exp Physiol. 2013 Nov;98(11):1608-19. doi: 10.1113/expphysiol.2013.073163. Epub 2013 Aug 16.
Hyaluronan (HA), the major glycosaminoglycan of the interstitial matrix, is heterogeneously distributed within the kidney. Using real-time RT-PCR, we tested the assumption that renal HA may be involved in the long-term effect of vasopressin on water reabsorption. The expression of the genes encoding hyaluronan synthase-2 (Has2), hyaluronidase-1 and hyaluronidase-2 (Hyal1 and Hyal2) was studied in the kidneys of Wistar Albino Glaxo (WAG) and homozygous vasopressin-deficient Brattleboro rats treated with the V2 receptor-selective vasopressin analogue dDAVP (100 μg (kg body wt)(-1), i.p., twice a day for 2 days). The Has2 mRNA content was the highest in the kidney papilla of the hydrated WAG and control Brattleboro rats, devoid of vasopressin. In WAG rats, dDAVP induced a considerable decrease in Has2 mRNA content in the papilla, with less pronounced changes in the cortex. The changes elicited by dDAVP in Brattleboro rats tended to be the same as in WAG rats, but weaker. In contrast to Has2, dDAVP treatment caused a significant increase in the Hyal1 and Hyal2 mRNA content in the renal papilla of WAG and Brattleboro rats. In rats of both strains, there was a good fit between Hyal1 and Hyal2 transcriptional levels and changes in hyaluronidase activity in the renal tissue. It is suggested that vasopressin is able to inhibit the synthesis of HA and concomitantly promote its degradation in the interstitium of the renal papilla, thereby facilitating water flow between elements of the renal countercurrent system. The implications for this effect are discussed in the context of the data in the literature.
透明质酸（HA）是细胞外基质中的主要糖胺聚糖，在肾脏中呈不均匀分布。我们使用实时 RT-PCR 测试了假设，即肾 HA 可能参与了血管加压素对水重吸收的长期作用。研究了编码透明质酸合酶-2（Has2）、透明质酸酶-1 和透明质酸酶-2（Hyal1 和 Hyal2）的基因在接受 V2 受体选择性血管加压素类似物 dDAVP（100μg(kg 体重)(-1)，腹腔内，每天两次，共 2 天）治疗的 Wistar Albino Glaxo（WAG）和同源性血管加压素缺乏 Brattleboro 大鼠肾脏中的表达。在水合的 WAG 和对照 Brattleboro 大鼠的肾脏乳头中，Has2 mRNA 含量最高，没有血管加压素。在 WAG 大鼠中，dDAVP 诱导 Has2 mRNA 含量在乳头中明显降低，皮质中变化不明显。dDAVP 在 Brattleboro 大鼠中引起的变化倾向于与 WAG 大鼠相同，但较弱。与 Has2 相反，dDAVP 处理导致 WAG 和 Brattleboro 大鼠肾脏乳头中 Hyal1 和 Hyal2 mRNA 含量显著增加。在两种大鼠中，Hyal1 和 Hyal2 转录水平与肾组织中透明质酸酶活性的变化之间存在良好的拟合。这表明血管加压素能够抑制 HA 的合成，并同时促进其在肾乳头间质中的降解，从而促进肾逆流系统元件之间的水流。在讨论文献中的数据时，讨论了这种作用的意义。
