从大脑中提纯神经调节因子神经抑素

Purification of neurocatin, a neuroregulatory factor from brain.

作者信息

Yee D K, Pastuszko P, Pastuszko A, Wilson D F

机构信息

Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.

出版信息

Neurosci Lett. 1990 Jul 3;114(2):213-9. doi: 10.1016/0304-3940(90)90074-j.

DOI:10.1016/0304-3940(90)90074-j

PMID:2395532

Abstract

A neuroregulatory factor (neurocatin) has been isolated from bovine brain. Neurocatin is a powerful affector of catecholamine metabolism in synaptosomes isolated from rat brain, causing increased formation of norepinephrine (NE) and decreased formation of 3,4-dihydroxyphenylacetic acid (DOPAC). The ratio NE/DOPAC in synaptosomes has been used to measure the neurocatin content of samples during isolation. Neurocatin has now been purified to a single chromatographic peak by high-resolution HPLC and a preliminary amino acid content measured. It appears to be a small peptide with a molecular weight in the range of 2,000-2,500 Da.

摘要

一种神经调节因子（神经抑素）已从牛脑中分离出来。神经抑素是从大鼠脑部分离出的突触体中儿茶酚胺代谢的强效影响因子，可导致去甲肾上腺素（NE）生成增加，3,4-二羟基苯乙酸（DOPAC）生成减少。在分离过程中，突触体中NE/DOPAC的比值已被用于测量样品中的神经抑素含量。目前，神经抑素已通过高分辨率高效液相色谱法纯化至单一色谱峰，并测定了初步的氨基酸含量。它似乎是一种小肽，分子量在2000 - 2500道尔顿范围内。