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[具有疏水特性的聚合物制成的筏在器官培养中的应用——陈氏筏法的一种新改良]

[The use of rafts made of polymers with hydrophobic properties in organ culture--a new modification of Chen's raft method].

作者信息

Barabanov V M

出版信息

Ontogenez. 1990 May-Jun;21(3):315-8.

PMID:2395593
Abstract

A new modification of tissue culture technique implying the use of rafts of hydrophobic polymers is suggested. Polyethylene rafts appeared to be the most appropriate for tissue culture of chick pituitary. The rafts were prepared as disks with an opening in the center. Millipore filter carrying the explants was placed over the opening. The rafts had good buoyancy, remained on the surface of the culture media for 1-2 weeks and were reusable.

摘要

提出了一种组织培养技术的新改进方法，该方法意味着使用疏水性聚合物筏。聚乙烯筏似乎最适合鸡垂体的组织培养。筏被制备成中心有开口的圆盘。携带外植体的微孔滤膜放置在开口上方。这些筏具有良好的浮力，可在培养基表面停留1至2周且可重复使用。

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