Aihemaiti Maierhaba, Zhen Fan, Li Yuezhong, Aibaidoula Gulisimayi, Yimit Wusiman
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Wei Sheng Wu Xue Bao. 2013 May 4;53(5):470-7.
We screened aerobic bacteria with cellulolytic activity from ruminal fluid of sheep, cattle and camel in Xinjiang.
Fresh ruminal fluid was inoculated on sterilized sodium carboxymethylcellulose agar plates. Highly cellulolytic aerobic bacteria were screened out by using Congo red staining and liquid secondary screening culture media. The combination of morphological and biochemical test with 16SrDNA sequence analysis were used to classify the strains. Enzymatic activities of four strains with strong cellulose-decomposing abilities were studied under different culture conditions.
Out 84 isolated cellulolytic strains, 40 exhibited strong abilities in decomposing cellulose. They are including 37 Gram-negative isolates and 3 Gram-positive strains. Identification of these 40 strains shows that they belong to 11 species of 6 genera, 16 strains in Stenotrophomonas maltophilia, 10 Ochrobactrum, 5 Sphingobacterium, 3 Microbacterium, 3 Paracoccus and 2 Pseudomonas. The results of the enzymatic studies of four strains with strong cellulolytic abilities indicates that the strains have the best enzyme producing property when straw powder was chosen as the carbon source; the pH at 5.5 -6.0 and temperature at 37 degrees C.
The strains with highly cellulolytic abilities isolated from ruminal fluid show strong abilities in cellulose decomposition.
从新疆绵羊、牛和骆驼的瘤胃液中筛选具有纤维素分解活性的需氧菌。
将新鲜瘤胃液接种于灭菌的羧甲基纤维素钠琼脂平板上。通过刚果红染色和液体二次筛选培养基筛选出高纤维素分解活性的需氧菌。采用形态学、生化试验与16SrDNA序列分析相结合的方法对菌株进行分类。研究了4株纤维素分解能力较强的菌株在不同培养条件下的酶活性。
在分离出的84株纤维素分解菌株中，40株表现出较强的纤维素分解能力。其中包括37株革兰氏阴性菌和3株革兰氏阳性菌。对这40株菌株的鉴定表明，它们属于6个属的11个种，嗜麦芽窄食单胞菌16株，苍白杆菌10株，鞘氨醇杆菌5株，微杆菌3株，副球菌3株，假单胞菌2株。对4株纤维素分解能力较强的菌株进行酶学研究的结果表明，以秸秆粉为碳源时，菌株产酶性能最佳；pH值为5.5 - 6.0，温度为37℃。
从瘤胃液中分离出的高纤维素分解能力菌株具有较强的纤维素分解能力。
