[小鼠诱导多能干细胞单个克隆的心肌细胞分化]

[Cardiomyocyte differentiation of individual clones murine induced pluripotent stem cells].

作者信息

Malysheva S V, Budash H V, Bil'ko N M, Heschheller J

出版信息

Fiziol Zh (1994). 2013;59(3):10-7.

Abstract

Cardiomyocyte differentiation of certain clones of murine induced pluripotent stem cells (iPS) was estimated. iPS were obtained due to reprogramming of murine embryonic fibroblasts with transposon-based Sleeping beauty plasmids as sene delivery systems. Differentiation was performed in suspension culture and in attached to tissue-culture plates embryoid bodies (EBs). Ascorbic acid was applied as inductor. According to the obtained results, the differentiation was tenfold more effective in attached EBs. Ascorbic acid stimulated the generation of cardiomiocytes.

摘要

对某些小鼠诱导多能干细胞（iPS）克隆的心肌细胞分化进行了评估。通过使用基于转座子的睡美人质粒作为基因传递系统对小鼠胚胎成纤维细胞进行重编程，从而获得了iPS。分化在悬浮培养以及附着于组织培养板的胚状体（EB）中进行。使用抗坏血酸作为诱导剂。根据所获得的结果，在附着的EB中分化效率提高了十倍。抗坏血酸刺激了心肌细胞的生成。