Ray S, Chatterjee K, De D, Ghosh D
Andrology, Endocrinology and Molecular Medicine Laboratory, Department of Bio-Medical Laboratory Science and Management (UGC Innovative Programme Funded Department), Vidyasagar University, Midnapore, West Bengal, India; Department of Gynaecology and Obstetrics, Midnapore Medical College and Hospital, Midnapore, West Bengal, India.
Andrologia. 2014 Aug;46(6):659-71. doi: 10.1111/and.12133. Epub 2013 Aug 19.
Increase in male sexual dysfunction, and its treatment with conventional aphrodisiac drugs with side effects lead to investigate the spermatogenesis and androgenesis augmentative efficacy of hydromethanolic (40 : 60) extract of root of Chlorophytum borivilianum (family - Liliaceae) against cyproterone acetate-induced subfertility in Wistar strain male albino rat. For this purpose, experimental rats were divided into three treatment groups: vehicle (received distilled water), cyproterone acetate (gastric intubation at 250 mg kg(-1) twice daily for 35 days) and cyproterone acetate plus root extract of C. borivilianum (gastric intubation at 250 mg kg(-1) plus 400 mg kg(-1) with an interval of 20 min twice daily for 35 days). After 35-day treatment, all rats were euthanised. Reproductive deviations towards negative side were investigated by screening the spermatogenic and steroidogenic biosensors. Oxidative stress profile in reproductive organs and sperm pellet was evaluated by biochemical assessment of antioxidative enzyme activities and level of end products of the lipid peroxidation. Apoptosis profile was evaluated by Western blot study, TUNEL assay and DNA fragmentation study of testicular tissues. Evaluation of toxicity profile was included for experimental investigation. After cyproterone acetate treatment, the pituitary-testicular axis was deviated towards the negative side and its tuning system was affected by oxidative stress and apoptosis-mediated process, which reduced the quality of semen and finally led to subfertility. Co-administration of C. borivilianum root extract enhanced male reproductive potentiality and prevented the negative deviations after the treatment with cyproterone acetate by means of increasing oxidative defence and maintaining homeostasis in testicular apoptosis process.
雄性性功能障碍的增加以及使用有副作用的传统壮阳药物进行治疗，促使人们研究波路豆根（百合科）水甲醇（40:60）提取物对醋酸环丙孕酮诱导的Wistar品系雄性白化大鼠亚生育能力的精子发生和雄激素生成增强功效。为此，将实验大鼠分为三个治疗组：载体组（接受蒸馏水）、醋酸环丙孕酮组（每天两次胃内插管250 mg kg⁻¹，持续35天）和醋酸环丙孕酮加波路豆根提取物组（每天两次胃内插管250 mg kg⁻¹加400 mg kg⁻¹，间隔20分钟，持续35天）。经过35天的治疗后，所有大鼠均被安乐死。通过筛选生精和类固醇生成生物传感器来研究向负面的生殖偏差。通过对抗氧化酶活性和脂质过氧化终产物水平的生化评估来评估生殖器官和精子沉淀中的氧化应激情况。通过蛋白质印迹研究、TUNEL检测和睾丸组织的DNA片段化研究来评估细胞凋亡情况。实验研究包括毒性情况评估。醋酸环丙孕酮治疗后，垂体 - 睾丸轴向负面偏离，其调节系统受到氧化应激和凋亡介导过程的影响，这降低了精液质量并最终导致亚生育能力。波路豆根提取物的联合给药通过增强氧化防御和维持睾丸细胞凋亡过程中的内环境稳定，提高了雄性生殖潜力并预防了醋酸环丙孕酮治疗后的负面偏差。
