Centre for Research in Biosciences, Dept. of Applied Sciences, Faculty of Health and Life Sciences, Univ. of the West of England, Bristol, Frenchay Campus, Coldharbour Lane, Bristol, BS16 1QY, UK.
J Food Sci. 2013 Aug;78(8):C1130-5. doi: 10.1111/1750-3841.12191.
This study investigated the role of lipogenic enzyme expression in breed-specific fat deposition in pigs.
(i) determine effect of breed on the relative abundance of the key lipogenic enzymes stearoyl-CoA desaturase (SCD), delta-6 desaturase (Δ6D), and fatty acid synthase (FAS) in pig subcutaneous adipose tissue. (ii) Investigate breed-specific relationships between lipogenic enzyme abundance and fatty acid composition. Large White × Piétrain, Piétrain, and Duroc × Piétrain pigs were used. Expression of SCD, Δ6D, and FAS was analyzed by Western blotting. Fatty acid composition was determined by gas chromatography. FAS protein in Large White × Piétrain pigs was similar to the Piétrain breed, but was significantly higher than Duroc × Piétrain. A positive relationship was found between FAS abundance and the saturated fatty acids (SFAs), for Large White × Piétrain pigs, but not for the other breeds. Δ6D was significantly higher in Large White × Piétrain compared with Duroc × Piétrain and Piétrain. This was accompanied by significantly higher total n-3 poly-unsaturated fatty acids (PUFAs) in the Large White × Piétrain when compared to the other breeds.
(i) increased subcutaneous adipose tissue SFA content in Large White × Piétrain pigs (but not Piétrain and Duroc × Piétrain) is related to increased abundance of FAS protein; (ii) high n-3 PUFA content in Large White × Piétrain pigs is related to activation of Δ6D protein synthesis; (iii) SCD and Δ6D abundance does not contribute to between-breed differences in MUFA and n-6 PUFA content of pig subcutaneous adipose tissue.
本研究旨在探讨脂肪生成酶表达在猪种特异性脂肪沉积中的作用。
（i）确定品种对猪皮下脂肪组织中关键脂肪生成酶硬脂酰辅酶 A 去饱和酶（SCD）、Δ6 去饱和酶（Δ6D）和脂肪酸合酶（FAS）相对丰度的影响。（ii）研究脂肪生成酶丰度与脂肪酸组成之间的品种特异性关系。使用长白猪×皮特兰猪、皮特兰猪和杜洛克×皮特兰猪。通过 Western blot 分析 SCD、Δ6D 和 FAS 的表达。通过气相色谱法测定脂肪酸组成。长白猪×皮特兰猪的 FAS 蛋白与皮特兰品种相似，但明显高于杜洛克×皮特兰猪。发现长白猪×皮特兰猪的 FAS 丰度与饱和脂肪酸（SFAs）呈正相关，但其他品种则没有。与杜洛克×皮特兰猪和皮特兰猪相比，长白猪×皮特兰猪的 Δ6D 显著升高。这伴随着长白猪×皮特兰猪总 n-3 多不饱和脂肪酸（PUFA）含量的显著增加，与其他品种相比。
（i）长白猪×皮特兰猪皮下组织 SFA 含量增加（而非皮特兰猪和杜洛克×皮特兰猪）与 FAS 蛋白丰度增加有关；（ii）长白猪×皮特兰猪高 n-3 PUFA 含量与 Δ6D 蛋白合成的激活有关；（iii）SCD 和 Δ6D 丰度与猪皮下脂肪组织 MUFA 和 n-6 PUFA 含量的品种间差异无关。
